PMID: 8590031Aug 1, 1995Paper

How is pheromone specificity encoded in proteins?

Chemical Senses
G D PrestwichS M LaForest

Abstract

Pheromone specificity in the Lepidoptera is encoded in protein components of the antennal sensillum lymph and dendritic membrane. In this paper, we highlight recent work on the molecular determinants of pheromone binding affinity of pheromone binding proteins (PBPs) of three genera. First, we describe new cDNA sequences for Lymantria dispar (Lymantriidae) and Agrotis segetum (Noctuidae). These data enrich the conclusions derived from our functional studies. Secondly, we indicate how preparation of multimilligram quantities of the recombinant PBP 'Apol-3' (originally from Antheraea polyphemus) has provided a platform (i) to determine the ligand binding sites using photoaffinity labeling, (ii) to conduct structural analysis by CD and NMR, and (iii) to measure binding affinities using a new binding assay. Thirdly, we describe the use of expression-cassette PCR technology to prepare two related PBPs from Antheraea perneyi to test binding affinities of naturally-occurring homologous PBPs. Our results support a model in which ligand specificity for chain length, double bond position, and terminal functionality is partially encoded in the PBPs. We propose that the final decoding is accomplished when the PBP-pheromone complex activates...Continue Reading

Citations

Jul 18, 2000·Archives of Insect Biochemistry and Physiology·J F PicimbonP Pelosi
Oct 23, 1998·Invertebrate Neuroscience : in·J KriegerH Breer
Aug 22, 2009·Journal of Comparative Physiology. A, Neuroethology, Sensory, Neural, and Behavioral Physiology·Karl-Ernst Kaissling
Mar 1, 1996·Bioorganic & Medicinal Chemistry·G D Prestwich
Aug 17, 2005·Insect Biochemistry and Molecular Biology·David AbrahamJean-François Picimbon
May 10, 2000·Insect Biochemistry and Molecular Biology·F E CallahanA K Mattoo
Oct 5, 2001·Insect Biochemistry and Molecular Biology·J F PicimbonH Breer
Jul 12, 2002·Insect Biochemistry and Molecular Biology·J F Picimbon, C Gadenne
Jan 18, 2003·Insect Biochemistry and Molecular Biology·R D NewcombD R Greenwood
May 1, 1997·Insect Biochemistry and Molecular Biology·L Feng, G D Prestwich
Nov 18, 1998·Insect Biochemistry and Molecular Biology·M Maïbèche-CoisnéP Nagnan-Le Meillour
May 13, 1999·Insect Biochemistry and Molecular Biology·C S Willett, R G Harrison
Sep 6, 2002·Genome Research·Daria S Hekmat-ScafeMark A Tanouye
Dec 1, 1995·European Journal of Biochemistry·J FeixasA Guerrero
Feb 4, 1999·Annals of the New York Academy of Sciences·P Pelosi
Feb 4, 1999·Annals of the New York Academy of Sciences·D B Kurtz, T L White
Jan 1, 1997·Annual Review of Neuroscience·J G Hildebrand, G M Shepherd
Jul 23, 2013·International Journal of Molecular Sciences·Weiwei ZhengHongyu Zhang
Dec 22, 1999·Biochemical and Biophysical Research Communications·A ScaloniP Pelosi
Nov 17, 2004·Proceedings of the National Academy of Sciences of the United States of America·Takeshi SakuraiTakaaki Nishioka
Mar 19, 2015·Insect Biochemistry and Molecular Biology·Richard G VogtJing-Jiang Zhou
Apr 21, 2009·Insect Biochemistry and Molecular Biology·Quan-Yong JiangLong Zhang
Nov 4, 2008·Insect Biochemistry and Molecular Biology·Patricia AcínCarmen Quero
Jan 22, 2008·Insect Biochemistry and Molecular Biology·Teresa C F AssumpçãoJosé M C Ribeiro
Oct 10, 2006·Structure·Frauke GräterHelmut Grubmüller
Oct 10, 2006·Structure·Frauke GräterHelmut Grubmüller
Mar 26, 2013·Biochemical and Biophysical Research Communications·Fang-Fang ZengMan-Qun Wang
Dec 21, 2000·Insect Molecular Biology·L M FieldL J Wadhams

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