How Kaposi's sarcoma-associated herpesvirus stably transforms peripheral B cells towards lymphomagenesis

Proceedings of the National Academy of Sciences of the United States of America
Aurélia FaureBill Sugden

Abstract

Primary effusion lymphomas (PELs) are causally associated with Kaposi's sarcoma-associated herpesvirus (KSHV) and 86% of PELs are coinfected with Epstein-Barr virus (EBV). Understanding how PELs develop has been impaired by the difficulty of infecting B cells with KSHV in vitro, and the inability of KSHV to transform them. We show that EBV supports an optimal coinfection of 2.5% of peripheral B cells by KSHV. This coinfection requires 1 or more transforming genes of EBV but not entry into KSHV's lytic cycle. We demonstrate that dually infected B cells are stably transformed in vitro and show that while both viruses can be maintained, different cells exhibit distinct, transformed properties. Transformed cells that grow to predominate in a culture express increased levels of most KSHV genes and differentially express a subset of cellular genes, as do bona fide PEL cells. These dually infected peripheral B cells are thus both stably transformed and allow in vitro molecular dissection of early steps in the progression to lymphomagenesis.

References

Sep 1, 1977·Journal of Virology·B Sugden, W Mark
Jul 1, 1977·The Journal of General Virology·F L GrahamR Nairn
Aug 1, 1992·Applied and Environmental Microbiology·F J van KuppeveldW J Melchers
Nov 1, 1972·The Journal of General Virology·D J Moss, J H Pope
Oct 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·G MillerM Lipman
May 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·M RabsonG Miller
Nov 1, 1995·The Journal of Experimental Medicine·P GarroneJ Banchereau
Dec 10, 1996·Proceedings of the National Academy of Sciences of the United States of America·J J RussoP S Moore
Jul 8, 1998·Proceedings of the National Academy of Sciences of the United States of America·H J DelecluseW Hammerschmidt
May 27, 1999·Seminars in Cancer Biology·E Cesarman, D M Knowles
Jul 14, 2000·AIDS·D J BlackbournJ A Levy
Nov 16, 2002·Science·Malini ChatterjeePatrick S Moore
May 14, 2003·Journal of Virology·Jill T BechtelDon Ganem
Apr 7, 2004·The Journal of Experimental Medicine·Ilaria GuasparriEthel Cesarman
May 28, 2004·Journal of Virology·Rafael E LunaKonstantin G Kousoulas
Oct 4, 2005·Proceedings of the National Academy of Sciences of the United States of America·Aravind SubramanianJill P Mesirov
May 25, 2007·The Oncologist·Yi-Bin ChenEphraim Hochberg
Jan 24, 2008·PloS One·Martina WiesnerAndreas Moosmann
Mar 14, 2008·Journal of Virology·Giovanna RappoccioloCharles R Rinaldo
Jul 22, 2008·Current Topics in Microbiology and Immunology·R F Kalejta
Aug 30, 2008·Journal of Virological Methods·Seung-Min YooMyung-Shin Lee
Nov 3, 2010·Advances in Cancer Research·David Vereide, Bill Sugden
Nov 23, 2010·Blood·David T Vereide, Bill Sugden
Jan 20, 2011·The Journal of Clinical Investigation·Lynn M HassmanDean H Kedes

❮ Previous
Next ❯

Citations

Aug 1, 2019·Proceedings of the National Academy of Sciences of the United States of America·Aurélia FaureBill Sugden
Feb 20, 2020·The Journal of Infectious Diseases·Peter O OluochAnn M Moormann
Jul 29, 2020·Viruses·Monika A ZelazowskaLaurie T Krug
Dec 22, 2020·Frontiers in Cellular and Infection Microbiology·Farizeh Aalam, Jennifer Totonchy
Apr 13, 2021·Frontiers in Cellular and Infection Microbiology·Christian Münz
Jun 22, 2021·Tumour Virus Research·Marta Maria Gaglia
Aug 11, 2021·Viruses·Yessica Sánchez-Ponce, Ezequiel M Fuentes-Pananá

❮ Previous
Next ❯

Datasets Mentioned

BETA
PRJNA362820

Methods Mentioned

BETA
PCR
flow cytometry
fluorescence
irradiate
fluorescence microscopy
FACS
RNA-seq
PCA
dissection

Software Mentioned

Mstat
apeglm
DESeq2
SDS
Adobe Photoshop
FLowJo
AxioVision
MSigDB
GSEA
repseqio

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.