May 21, 2003

How much effort is required to isolate nuclear microsatellites from plants?

Molecular Ecology
J SquirrellWayne Powell

Abstract

The attributes of codominance, reproducibility and high resolution have all contributed towards the current popularity of nuclear microsatellites as genetic markers in molecular ecological studies. One of their major drawbacks, however, is the development phase required to obtain working primers for a given study species. To facilitate project planning, we have reviewed the literature to quantify the workload involved in isolating nuclear microsatellites from plants. We highlight the attrition of loci at each stage in the process, and the average effort required to obtain 10 working microsatellite primer pairs.

Mentioned in this Paper

Excessive Attrition of Teeth
Short Tandem Repeat
Molecular Probe Techniques
Genetic Markers
Isolate compound
Oligonucleotide Primers
cDNA Library
Tetranucleotide Repeats
Tooth Attrition
Genetic Marker Expression

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