PMID: 26405Apr 27, 1978

How much is secondary structure responsible for resistance of double-stranded RNA to pancreatic ribonuclease A?

Biochimica Et Biophysica Acta
M Libonati, M Palmieri

Abstract

1. Double-stranded f2 sus11 or Qbeta RNAs, resistant to bovine pancreatic RNAase A in 0.15 M NaCl/0.015 M sodium citrate (SSC), are quickly and completely degraded at 10-fold lower ionic strength (0.1 X SSC) under otherwise similar conditions. At this ionic strength the secondary structure of double-stranded RNA is maintained, as judged by the following: (a) the unchanged resistance of double-stranded RNA and DNA, under similar low ionic strength conditions, to nuclease S1 from Aspergillus oryzae, in contrast with the sensitivity of the corresponding denatured nucleic acids to this enzyme, specific for single-stranded RNA and DNA; (b) the co-operative pattern of the thermal-transition profile of double-stranded RNA (with a Tm of 89 degrees C) in 0.1 X SSC. 2. Whereas in SSC bovine seminal RNAase (RNAase BS-1) and whale pancreatic RNAase show an activity on double-stranded RNA significantly higher than that of RNAase A, in 0.1 X SSC the activity of the latter enzyme on this substrate becomes distinctly higher than that of RNAase BS-1, and similar to that of whale RNAase. 3. From these results it is deduced that the secondary structure is probably not the only nor the most important variable in determining the susceptibility doub...Continue Reading

References

Mar 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·P Douzou, P Maurel
Feb 10, 1976·Biochemistry·D WangS Moore
Oct 1, 1963·Proceedings of the National Academy of Sciences of the United States of America·G FELSENFELDP H VONHIPPEL

Citations

Jul 17, 2007·Biochemical and Biophysical Research Communications·Punyatirtha DeyJanendra K Batra
Aug 26, 1980·Biochimica Et Biophysica Acta·S SorrentinoM Libonati
Jan 1, 1981·Scandinavian Journal of Immunology·J JonssonR Norberg
May 1, 1982·European Journal of Biochemistry·S SorrentinoM Libonati

Related Concepts

Aspergillus oryzae
3'-CMP
DNA, Double-Stranded
Hot Temperature
Hydrogen-Ion Concentration
DNA Conformation
RNA Denaturation
Nucleotides, Cyclic
Osmolality
Pancreas

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