PMID: 7542015Mar 1, 1995Paper

How the developing septo-preoptic medical basal hypothalamus stimulates the development of placode-derived LHRH neurons

Archives of Histology and Cytology
S DaikokuY Natori

Abstract

We examined the effects of the developing cerebral cortex (CC) and septo-preoptic medial basal hypothalamus (S-MBH) on the development of LHRH neurons in vitro. The serum-free basal culture medium (BCM) was supplemented with CC or S-MBH extracts prepared from 18.5-day-old embryos or from 2-day-old newborns, and the olfactory placode (NAP) of 12-day-old embryos was cultured. The migration of LHRH neurons was found on Day 3 in the cultures supplemented with the embryonic S-MBH extract (Group 3), where the cell development proceeded showing a numerical increase of the cells and the elongation of neurites. In cultures supplemented with the newborn S-MBH extract (Group 5), the cell development was less intensive in comparison with that of Group 3, while in cultures which had no brain extracts (Group 1), the neurons failed to survive a long term culture. The effects of the CC were less than of S-MBH extracts. Analysis of the protein composition of the extracts by electrophoretic and immunoblotting examinations demonstrated a protein spot of 70-kD in the embryonic S-MBH extract. Because the protein spot was identified to be alpha-fetoprotein (AFP), we further examined the effects of AFP. When the anti-AFP immunoglobulin was added to t...Continue Reading

Citations

Jul 20, 2002·Frontiers in Neuroendocrinology·S Wray
Mar 31, 1998·The Journal of Comparative Neurology·S Daikoku, I Koide
Sep 25, 2004·Journal of Neurobiology·Melonie J Storan, Brian Key

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