How Tissue Mechanical Properties Affect Enteric Neural Crest Cell Migration

Scientific Reports
N R ChevalierV Fleury

Abstract

Neural crest cells (NCCs) are a population of multipotent cells that migrate extensively during vertebrate development. Alterations to neural crest ontogenesis cause several diseases, including cancers and congenital defects, such as Hirschprung disease, which results from incomplete colonization of the colon by enteric NCCs (ENCCs). We investigated the influence of the stiffness and structure of the environment on ENCC migration in vitro and during colonization of the gastrointestinal tract in chicken and mouse embryos. We showed using tensile stretching and atomic force microscopy (AFM) that the mesenchyme of the gut was initially soft but gradually stiffened during the period of ENCC colonization. Second-harmonic generation (SHG) microscopy revealed that this stiffening was associated with a gradual organization and enrichment of collagen fibers in the developing gut. Ex-vivo 2D cell migration assays showed that ENCCs migrated on substrates with very low levels of stiffness. In 3D collagen gels, the speed of the ENCC migratory front decreased with increasing gel stiffness, whereas no correlation was found between porosity and ENCC migration behavior. Metalloprotease inhibition experiments showed that ENCCs actively degraded ...Continue Reading

References

Feb 1, 1980·The American Journal of Anatomy·I J Allan, D F Newgreen
Jun 27, 2000·Biophysical Journal·C M LoY L Wang
Jul 25, 2000·Mechanisms of Development·R Perris, D Perissinotto
Jan 11, 2001·Developmental Biology·H M YoungD F Newgreen
Dec 25, 2002·Neuroreport·Lisa A FlanaganPaul A Janmey
Feb 11, 2003·Journal of Structural Biology·Guy CoxMark D Gorrell
Jun 9, 2004·Developmental Biology·H M YoungP M Whitington
Jul 21, 2004·Current Opinion in Genetics & Development·Eric Brouzés, Emmanuel Farge
Dec 2, 2004·Cell Motility and the Cytoskeleton·Tony YeungPaul A Janmey
Oct 4, 2005·Developmental Biology·Noah R Druckenbrod, Miles L Epstein
Nov 19, 2005·Science·Dennis E DischerYu-Li Wang
May 3, 2006·Developmental Biology·Laure StanchinaNadege Bondurand
Jul 13, 2006·The Anatomical Record. Part A, Discoveries in Molecular, Cellular, and Evolutionary Biology·Bridget R Southwell
Aug 23, 2006·Cell·Adam J EnglerDennis E Discher
Oct 19, 2006·Current Opinion in Cell Biology·Manuel Théry, Michel Bornens
Feb 28, 2007·Investigative Ophthalmology & Visual Science·Naoyuki MorishigeJames V Jester
Jul 7, 2007·Methods in Cell Biology·Lance Davidson, Ray Keller
Feb 1, 2008·Journal of Molecular Biology·F GobeauxE Belamie
Jul 19, 2008·Pediatric Research·Allan M Goldstein, Nandor Nagy
Oct 1, 2008·Optics Express·Christophe OdinYann Le Grand
Feb 28, 2009·The International Journal of Developmental Biology·Richard B Anderson
Jul 21, 2009·Current Opinion in Cell Biology·Rebeca M Tenney, Dennis E Discher
Sep 4, 2009·The Journal of Biological Chemistry·Geert van TeteringMarc Vooijs
Sep 25, 2009·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·R HottaH M Young
Jan 29, 2010·Annals of Biomedical Engineering·Ramji T VenkatasubramanianJohn C Bischof
Jun 4, 2010·Current Protocols in Cell Biology·Justin R Tse, Adam J Engler
Aug 19, 2010·The EMBO Journal·Ekaterina Papusheva, Carl-Philipp Heisenberg
Dec 18, 2010·Optics Express·Thomas GuilbertDominique Guyader
Apr 20, 2011·Burns : Journal of the International Society for Burn Injuries·Alice C-H ChenJames R McMillan
Aug 5, 2011·Nature·Thierry SavinClifford J Tabin

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Citations

Feb 22, 2017·Acta Biomaterialia·Marcus BinnerCarsten Werner
Jun 1, 2017·EMBO Molecular Medicine·Samuele NegroCesare Montecucco
Oct 9, 2019·Journal of the Royal Society, Interface·Diana KhalipinaNicolas R Chevalier
Oct 18, 2019·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Rasa GiniūnaitėPhilip K Maini
Jun 25, 2020·Advanced Materials·John M ViolaAlex J Hughes
Jun 28, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Ming FuAnkush Gosain
Sep 27, 2018·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Niamh C NowlanCeleste Nelson
Sep 27, 2018·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Jacob M Jaslove, Celeste M Nelson
Aug 15, 2019·Annual Review of Cell and Developmental Biology·Alessandro MongeraOlivier Pourquié
Jul 12, 2020·Journal of Cell Science·Pardis PakshirBoris Hinz
May 30, 2020·Development·David A Cruz Walma, Kenneth M Yamada
Apr 23, 2020·International Journal of Molecular Medicine·Antonios KostourosJohn Tsiaoussis
Jan 6, 2021·Cells·Verena Pawolski, Mirko H H Schmidt
Jun 25, 2021·Communications Biology·Nicolas R ChevalierSylvie Dufour
Aug 7, 2021·Scientific Reports·Georgina Navoly, Conor J McCann
Jan 15, 2017·Seminars in Cell & Developmental Biology·Nandor Nagy, Allan M Goldstein

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Datasets Mentioned

BETA
GM6001

Methods Mentioned

BETA
atomic force microscopy
AFM
rheology

Software Mentioned

Photoshop
MetaMorph
ImageJ
Fiji

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