Microbial enzymes have been used in a large number of fields, such as chemical, agricultural and biopharmaceutical industries. The enzyme production rate and yield are the main factors to consider when choosing the appropriate expression system for the production of recombinant proteins. Recombinant enzymes have been expressed in bacteria (e.g., Escherichia coli, Bacillus and lactic acid bacteria), filamentous fungi (e.g., Aspergillus) and yeasts (e.g., Pichia pastoris). The favorable and very advantageous characteristics of these species have resulted in an increasing number of biotechnological applications. Bacterial hosts (e.g., E. coli) can be used to quickly and easily overexpress recombinant enzymes; however, bacterial systems cannot express very large proteins and proteins that require post-translational modifications. The main bacterial expression hosts, with the exception of lactic acid bacteria and filamentous fungi, can produce several toxins which are not compatible with the expression of recombinant enzymes in food and drugs. However, due to the multiplicity of the physiological impacts arising from high-level expression of genes encoding the enzymes and expression hosts, the goal of overproduction can hardly be ac...Continue Reading
The influence of ribosome-binding-site elements on translational efficiency in Bacillus subtilis and Escherichia coli in vivo
Translational fusions with fragments of the trpE gene improve the expression of a poorly expressed heterologous gene in Escherichia coli
Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter
Metabolic engineering of Escherichia coli to enhance recombinant protein production through acetate reduction
Process economics of animal cell and bacterial fermentations: a case study analysis of tissue plasminogen activator
Overexpression and rapid purification of rabbit fast skeletal troponin I from Escherichia coli: effect of different promoters, host strains, and culture conditions
A gene cassette for adapting Escherichia coli strains as hosts for att-Int-mediated rearrangement and pL expression vectors
The 19-residue pro-peptide of staphylococcal nuclease has a profound secretion-enhancing ability in Escherichia coli
High-level misincorporation of lysine for arginine at AGA codons in a fusion protein expressed in Escherichia coli
High-level expression of Bacillus thuringiensis phosphatidylinositol-specific phospholipase C by the Bacillus brevis host-vector system
A tightly regulated high level expression vector that utilizes a thermosensitive lac repressor: production of the human T cell receptor V beta 5.3 in Escherichia coli
Isolation of the Pichia pastoris glyceraldehyde-3-phosphate dehydrogenase gene and regulation and use of its promoter
Analysis of a Streptococcus pneumoniae gene encoding signal peptidase I and overproduction of the enzyme
Modification of ricin A chain, by addition of endoplasmic reticulum (KDEL) or Golgi (YQRL) retention sequences, enhances its cytotoxicity and translocation
Tight transcriptional control mechanism ensures stable high-level expression from T7 promoter-based expression plasmids
Indirect regulation of translational termination efficiency at highly expressed genes and recoding sites by the factor recycling function of Escherichia coli release factor RF3
An expression system matures: a highly efficient and cost-effective process for phytase production by recombinant strains of Hansenula polymorpha
The use of multi-parameter flow cytometry to compare the physiological response of Escherichia coli W3110 to glucose limitation during batch, fed-batch and continuous culture cultivations
A protein disulfide isomerase gene fusion expression system that increases the extracellular productivity of Bacillus brevis.
Overcoming codon bias: a method for high-level overexpression of Plasmodium and other AT-rich parasite genes in Escherichia coli
A mutation in secY that causes enhanced SecA insertion and impaired late functions in protein translocation.
Use of modified BL21(DE3) Escherichia coli cells for high-level expression of recombinant peanut allergens affected by poor codon usage
Primary structure and expression of peroxisomal acetylspermidine oxidase in the methylotrophic yeast Candida boidinii
High expression of the penicillin G acylase gene (pac) from Bacillus megaterium UN1 in its own pac minus mutant
Coupling the T7 A1 promoter to the runaway-replication vector as an efficient method for stringent control and high-level expression of lacZ
Codon bias at the 3'-side of the initiation codon is correlated with translation initiation efficiency in Escherichia coli
DegP-coexpression minimizes inclusion-body formation upon overproduction of recombinant penicillin acylase in Escherichia coli
A spontaneous runaway vector for production-scale expression of bovine somatotropin from Escherichia coi
Heterologous expression of the Saccharomyces cerevisiae PGU1 gene in Schizosaccharomyces pombe yields an enzyme with more desirable properties for the food industry.
Effects of inactivation and constitutive expression of the unfolded- protein response pathway on protein production in the yeast Saccharomyces cerevisiae.
Optimization of the extracellular production of a bacterial phytase with Escherichia coli by using different fed-batch fermentation strategies
Hsp27 suppresses the formation of inclusion bodies induced by expression of R120G alpha B-crystallin, a cause of desmin-related myopathy
High-level production of hyperthermophilic cellulase in the Bacillus brevis expression and secretion system
DnaK and DnaJ facilitated the folding process and reduced inclusion body formation of magnesium transporter CorA overexpressed in Escherichia coli
A facile and efficient method to achieve LacZ overproduction by the expression vector carrying the thermoregulated promoter and plasmid copy number
Improved production of heterologous proteins by a glucose repression-defective mutant of Kluyveromyces lactis.
Significantly improving the yield of recombinant proteins in Bacillus subtilis by a novel powerful mutagenesis tool (ARTP): Alkaline α-amylase as a case study
A constitutive expression system for cellulase secretion in Escherichia coli and its use in bioethanol production
Microbial diversity in various types of paper mill sludge: identification of enzyme activities with potential industrial applications
ATGme: Open-source web application for rare codon identification and custom DNA sequence optimization
Molecular and catalytic properties of fungal extracellular cellobiose dehydrogenase produced in prokaryotic and eukaryotic expression systems
Use of a Sec signal peptide library from Bacillus subtilis for the optimization of cutinase secretion in Corynebacterium glutamicum
Synthetic repetitive extragenic palindromic (REP) sequence as an efficient mRNA stabilizer for protein production and metabolic engineering in prokaryotic cells
Characterization, expression and application of a zearalenone degrading enzyme from Neurospora crassa
Significantly enhancing recombinant alkaline amylase production in Bacillus subtilis by integration of a novel mutagenesis-screening strategy with systems-level fermentation optimization
Screening of a genome-reduced Corynebacterium glutamicum strain library for improved heterologous cutinase secretion.
Implications of endophytic microbiota in Camellia sinensis: a review on current understanding and future insights.
Pseudomonas mRNA 2.0: Boosting Gene Expression Through Enhanced mRNA Stability and Translational Efficiency
Development of L-Asparaginase Biobetters: Current Research Status and Review of the Desirable Quality Profiles
Characterization of a novel thermostable carboxylesterase from thermoalkaliphilic bacterium Bacillus thermocloaceae
A comprehensive review on fungal endophytes and its dynamics on Orchidaceae plants: current research, challenges, and future possibilities
The industrial yeast Pichia pastoris is converted from a heterotroph into an autotroph capable of growth on CO2
Enhancing extracellular protein production in Escherichia coli by deleting the d-alanyl-d-alanine carboxypeptidase gene dacC
Reciprocal relation between reporter gene transcription and translation efficiency in fission yeast.
Enzymes revolutionize the bioproduction of value-added compounds: From enzyme discovery to special applications
Bacterial sialyltransferases and their use in biocatalytic cascades for sialo-oligosaccharide production
Enhancement of solubility, purification and inclusion-bodies-refolding of an active pectin lyase from Penicillium occitanis expressed in Escherichia coli
WITHDRAWN: Efficient enzyme formulation promotes Leloir glycosyltransferases for glycoside synthesis.
Continuous process technology for glucoside production from sucrose using a whole cell-derived solid catalyst of sucrose phosphorylase.
Bioinformatics in Biomedicine
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