How to Generate Non-Mosaic CRISPR/Cas9 Mediated Knock-In and Mutations in F0 Xenopus Through the Host-Transfer Technique

Methods in Molecular Biology
Emmanuel Tadjuidje, Sang-Wook Cha

Abstract

We have taken advantage of the well-established oocyte host transfer technique to optimize a method for CRISPR editing of Xenopus that provides an efficient non-mosaic targeted insertion of small DNA fragment through homology-directed repair mechanism.

Citations

Nov 30, 2019·Frontiers in Cell and Developmental Biology·Jeehyun JeonYong-Kook Kang

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