HoxA cluster is haploinsufficient for activity of hematopoietic stem and progenitor cells

Experimental Hematology
Charles-Étienne Lebert-GhaliJanet Bijl

Abstract

Functional compensation between homeodomain proteins has hindered the ability to unravel their role in hematopoiesis using single gene knockouts. Because HoxB genes are dispensable for hematopoiesis, and most HoxA genes are expressed an order of magnitude higher than other cluster genes in hematopoietic stem cell (HSC)-enriched populations, we hypothesize that maintenance of HoxA cluster expression is important for adult hematopoiesis and that global decrease of HoxA gene expression levels affects steady-state hematopoiesis. Expression levels of HoxA cluster genes have been determined in primitive hematopoietic populations derived from adult mice using quantitative reverse transcriptase polymerase chain reaction. Furthermore, the functional effect of single allelic deletion of the entire HoxA cluster on hematopoietic cells was analyzed by competitive repopulation assays using HoxA(+/-) mice. We show that the HoxA cluster is predominantly expressed in long-term HSCs and that expression declines with progression to short-term HSCs and early progenitors in a quantifiable manner. Monoallelic deletion of the HoxA cluster caused a general increase in primitive hematopoietic cell populations, but a decrease in side populations. In add...Continue Reading

References

Mar 31, 1992·Biochemical and Biophysical Research Communications·I Vieille-GrosjeanG Courtois
Jul 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·M C MagliE Boncinelli
Nov 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·W F ShenH J Lawrence
Jun 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·R K HumphriesC J Eaves
Dec 6, 1994·Proceedings of the National Academy of Sciences of the United States of America·G SauvageauR K Humphries
Apr 25, 1993·Nucleic Acids Research·M P Scott
Jun 11, 1996·Proceedings of the National Academy of Sciences of the United States of America·M LaksoH Westphal
May 1, 1996·Stem Cells·H J LawrenceC Largman
Jan 15, 1997·Developmental Biology·F Chen, M R Capecchi
Dec 13, 2000·Molecular and Cellular Biology·U ThorsteinsdottirG Sauvageau
Sep 14, 2002·Science·Natalia B IvanovaIhor R Lemischka
Oct 9, 2002·Immunological Reviews·Kimberly J Payne, Gay M Crooks
Mar 24, 2007·Proceedings of the National Academy of Sciences of the United States of America·Michelle B BowieConnie J Eaves
Dec 31, 2008·Stem Cells and Development·Diego S VieyraMargaret A Goodell
Feb 5, 2010·Blood·Christian BachRobert K Slany
Mar 9, 2010·Cell Stem Cell·Grant A ChallenMargaret A Goodell
Mar 23, 2010·Developmental Biology·Nicolas Di-PoïDenis Duboule

❮ Previous
Next ❯

Citations

Jul 14, 2011·Stem Cells and Development·Marilaine FournierJanet J Bijl
Apr 1, 2011·Genome Research·Christopher P ArnoldChang-Zheng Chen
Jan 24, 2012·Stem Cells·Lesley M Forrester, Melany Jackson
Dec 7, 2013·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Nuala M MulgrewAlexander Thompson
Sep 3, 2013·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Mona HassawiJanet J Bijl
Jul 15, 2015·Stem Cells and Development·Marilaine FournierJanetta J Bijl
Jul 28, 2016·Stem Cells and Development·Fatemeh KamkarNaisana S Asli
Apr 10, 2017·Experimental Hematology·Charles-Étienne Lebert-GhaliJanet J Bijl
Apr 21, 2017·Oncotarget·Richard MorganHardev S Pandha
May 18, 2016·Nature Cell Biology·Diana R DouHanna K A Mikkola
Apr 13, 2019·Cancers·Monika PrimonRichard Morgan
Mar 18, 2020·Nature Communications·Lucia Cabal-HierroAndrew A Lane
Aug 11, 2021·Biochemical Society Transactions·Mays AbuhantashAlexander Thompson

❮ Previous
Next ❯

Related Concepts

Related Feeds

B cells: Gene Expression

B lymphocytes are white blood cells that play a role in the adaptive immune system by secreting antibodies. Here is the latest research on gene expression in B cells.

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.

Blood And Marrow Transplantation

The use of hematopoietic stem cell transplantation or blood and marrow transplantation (bmt) is on the increase worldwide. BMT is used to replace damaged or destroyed bone marrow with healthy bone marrow stem cells. Here is the latest research on bone and marrow transplantation.

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.