HOXA10 regulates endometrial GABAA {pi} receptor expression and membrane translocation.

American Journal of Physiology. Endocrinology and Metabolism
Homayoun Sadeghi, Hugh S Taylor

Abstract

Expression of the GABA(A) pi receptor has been described previously in the human endometrium in both luminal epithelium and stroma. Its expression is increased during decidualization in rodents and in the implantation window of human endometrium. Here we localized GABA pi subunit receptor protein in human endometrium and identified regulators of gene expression and activation. GABA(A) pi was localized to the cell surface, and expression increased during the window of embryo implantation in human endometrium. The well-differentiated human endometrial adenocarcinoma cell line Ishikawa was treated with progesterone and transfected with pcDNA-HOXA10, HOXA10 siRNA, or respective controls. GABA(A) pi receptor mRNA expression was evaluated by real-time RT-PCR. Protein expression and localization were evaluated using immunofluorescence. GABA(A) pi receptor mRNA expression was increased significantly after either progesterone treatment or HOXA10 transfection. Coadministration of progesterone along with HOXA10 transfection had no additional effect on the expression of GABA(A) pi receptor mRNA over either agent alone. Blocking HOXA10 expression with siRNA prevented progesterone-induced GABA(A) pi receptor mRNA expression. Additionally, ei...Continue Reading

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Citations

Mar 5, 2014·Human Reproduction Update·Ye Chun RuanHsiao Chang Chan
Jun 4, 2020·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Małgorzata SzczukoMaciej Ziętek
May 26, 2016·International Journal of Molecular Medicine·Junjie LuYi Tan

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