HOXA11 gene is hypermethylation and aberrant expression in gastric cancer

Cancer Cell International
Yinguo BaiShaoping Ji

Abstract

Aberrant DNA methylation is an acquired epigenetic alteration that serves as an alternative to genetic defects in the inactivation of tumor suppressor genes and other genes in diverse human cancers. Gastric carcinoma is one of the tumors with a high frequency of aberrant methylation in promoter region. Hence we investigated the promoter methylation status and expression level of HOXA11 gene which may involve in GC development. Thirty-two surgical excised gastric cancer specimens, twelve paired adjacent non-cancerous specimens and seven normal gastric mucosas were examined. The methylation status and expression level of HOXA11 gene were determined by bisulfite sequencing polymerase chain reaction (BSP), real-time polymerase chain reaction (RT-PCR) and immunohistochemistry (IHC) respectively. HOXA11 expression was knocked-down with siRNA to mimic HOXA11 gene hypermethylation and ability of cell proliferation and migration was determinate. In addition, we analyzed and correlated the findings with clinicopathological features. The methylation level of HOXA11 gene in gastric cancer tissues and adjacent non-cancerous tissues were higher than those in normal gastric mucosa (P < 0.05). The methylation level was higher in TNM III and IV...Continue Reading

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Citations

Jan 16, 2015·Epigenomics·Yingying CuiMingzhou Guo
Jul 28, 2016·Cancer Research and Treatment : Official Journal of Korean Cancer Association·Young-Bem SeChul-Kee Park
May 31, 2019·Epigenetics : Official Journal of the DNA Methylation Society·Youwei ZhangSanyuan Sun
Sep 3, 2016·Oncology Letters·Yingchao WangXiaojuan Ouyang
Jun 18, 2019·Oncology Reviews·Majid GhanavatSaeid Shahrabi

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Methods Mentioned

BETA
transfection
Reverse-PCR
PCR

Software Mentioned

CpG Island Searcher
SPSS

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