HOXA7, HOXA9, and HOXA10 are differentially expressed in clival and sacral chordomas

Scientific Reports
Daniela JägerKevin Mellert

Abstract

Chordomas are rare tumours of the bone arising along the spine from clivus to sacrum. We compared three chordoma cell lines of the clivus region including the newly established clivus chordoma cell line, U-CH14, with nine chordoma cell lines originating from sacral primaries by morphology, on genomic and expression levels and with patient samples from our chordoma tissue bank. Clinically, chordomas of the clivus were generally smaller in size at presentation and patients with sacral chordomas had more metastases and more often recurrent disease. All chordoma cell lines had a typical physaliphorous morphology and expressed brachyury, S100-protein and cytokeratin. By expression analyses we detected differentially expressed genes in the clivus derived cell lines as compared to the sacral cell lines. Among these were HOXA7, HOXA9, and HOXA10 known to be important for the development of the anterior-posterior body axis. These results were confirmed by qPCR. Immunohistologically, clivus chordomas had no or very low levels of HOXA10 protein while sacral chordomas showed a strong nuclear positivity in all samples analysed. This differential expression of HOX genes in chordomas of the clivus and sacrum suggests an oncofetal mechanism in...Continue Reading

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Citations

Feb 27, 2020·Der Pathologe·Susanne ScheiplBernadette Liegl
Jun 21, 2018·Medicine·Yibiao ZhouBin Zhang
Sep 18, 2020·Journal of Neuropathology and Experimental Neurology·Haiyin ZhengQi Zhang
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Datasets Mentioned

BETA
GSE95084
GSE68497

Methods Mentioned

BETA
surgical resection
xenograft
genotyping
electrophoresis
PCR
protein assay

Software Mentioned

SIFT
PHD
FATHMM
Biomedical Workbench
SNAP
Gene marker
GeneRead
SNP
Genespring
PANTHER

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