Hoxb8-Cre mice: A tool for brain-sparing conditional gene deletion.

Genesis : the Journal of Genetics and Development
Robert WitschiHanns Ulrich Zeilhofer

Abstract

The spinal cord is the first site of temporal and spatial integration of nociceptive signals in the pain pathway. Neuroplastic changes occurring at this site contribute critically to various chronic pain syndromes. Gene targeting in mice has generated important insights into these processes. However, the analysis of constitutive (global) gene-deficient mice is often hampered by confounding effects arising from supraspinal sites. Here, we describe a novel Cre mouse line that expresses the Cre recombinase under the transcriptional control of the Hoxb8 gene. Within the neural axis of these mice, Hoxb8-Cre expression is found in spinal cord neurons and glial cells, and in virtually all neurons of the dorsal root ganglia, but spares the brain apart from a few cells in the spinal trigeminal nucleus. The Hoxb8-Cre mouse line should be a valuable new tool for the in vivo analysis of peripheral and spinal gene functions in pain pathways.

References

Jan 24, 1992·Cell·W McGinnis, R Krumlauf
Apr 1, 1993·Developmental Biology·J Deschamps, M Wijgerde
May 16, 2001·Genesis : the Journal of Genetics and Development·R ConstienB Arnold
Jan 7, 2003·The EMBO Journal·Tony OosterveenJacqueline Deschamps
Feb 21, 2003·Current Opinion in Neurobiology·Amy W Helms, Jane E Johnson
Mar 30, 2004·Genesis : the Journal of Genetics and Development·Nitin AgarwalRohini Kuner
Mar 24, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Benjamin K YeeDetlev Boison
Jun 21, 2007·Nature Immunology·Marc Schmidt-Supprian, Klaus Rajewsky
Apr 24, 2008·Proceedings of the National Academy of Sciences of the United States of America·Jan C HolstegeJacqueline Deschamps

❮ Previous
Next ❯

Citations

Sep 21, 2013·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Jolly PaulHanns Ulrich Zeilhofer
Apr 26, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Hendrik WildnerCarmen Birchmeier
Nov 10, 2015·Nature Neuroscience·Seung-Hyun WooArdem Patapoutian
Aug 21, 2015·Neuron·Cedric PeirsRebecca P Seal
May 18, 2017·Nature Communications·Vojč Kocman, Janez Plavec
Jul 12, 2017·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Jimmy PengFrédéric Charron
Jun 13, 2018·The Journal of Clinical Investigation·Ming-Dong ZhangTomas Hökfelt
Dec 4, 2019·Nature Neuroscience·Mariko L Bennett, F Chris Bennett
Aug 15, 2018·Nature Communications·William T RalveniusHanns Ulrich Zeilhofer
Oct 12, 2018·Science Translational Medicine·Swetha E MurthyArdem Patapoutian

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.