HP1 links centromeric heterochromatin to centromere cohesion in mammals

EMBO Reports
Qi YiFangwei Wang

Abstract

Heterochromatin protein-1 (HP1) is a key component of heterochromatin. Reminiscent of the cohesin complex which mediates sister-chromatid cohesion, most HP1 proteins in mammalian cells are displaced from chromosome arms during mitotic entry, whereas a pool remains at the heterochromatic centromere region. The function of HP1 at mitotic centromeres remains largely elusive. Here, we show that double knockout (DKO) of HP1α and HP1γ causes defective mitosis progression and weakened centromeric cohesion. While mutating the chromoshadow domain (CSD) prevents HP1α from protecting sister-chromatid cohesion, centromeric targeting of HP1α CSD alone is sufficient to rescue the cohesion defects in HP1 DKO cells. Interestingly, HP1-dependent cohesion protection requires Haspin, an antagonist of the cohesin-releasing factor Wapl. Moreover, HP1α CSD directly binds the N-terminal region of Haspin and facilitates its centromeric localization. The need for HP1 in cohesion protection can be bypassed by centromeric targeting of Haspin or inhibiting Wapl activity. Taken together, these results reveal a redundant role for HP1α and HP1γ in the protection of centromeric cohesion through promoting Haspin localization at mitotic centromeres in mammalian...Continue Reading

References

Feb 5, 2000·Current Biology : CB·J F Smothers, S Henikoff
Oct 13, 2001·Science·P BernardR C Allshire
Jan 10, 2002·Nature Cell Biology·Nobuhiro NonakaYoshinori Watanabe
Apr 9, 2004·Nature Reviews. Molecular Cell Biology·Christèle Maison, Geneviève Almouzni
Aug 11, 2004·The Journal of Cell Biology·Mounia GuenatriGeneviève Almouzni
Nov 7, 2006·Developmental Cell·Jun DaiJonathan M G Higgins
Nov 23, 2006·Cell·Stephanie KuengJan-Michael Peters
Feb 27, 2008·Proceedings of the National Academy of Sciences of the United States of America·Thomas D BarberPhilip Hieter
Jul 4, 2008·Nature·Christian H HaeringKim Nasmyth
Aug 22, 2008·Nature·Yuya YamagishiYoshinori Watanabe
Oct 12, 2010·Science·Yuya YamagishiYoshinori Watanabe
Nov 30, 2010·Cell·Tomoko NishiyamaJan-Michael Peters
Jun 11, 2011·Current Biology : CB·John R DaumGary J Gorbsky
Oct 9, 2012·Cold Spring Harbor Perspectives in Biology·Jan-Michael Peters, Tomoko Nishiyama
Jan 5, 2013·Science·Le CongFeng Zhang
Jan 5, 2013·Science·Prashant MaliGeorge M Church
Aug 1, 2013·Proceedings of the National Academy of Sciences of the United States of America·Tomoko NishiyamaJan-Michael Peters
Nov 23, 2013·Current Biology : CB·Gary J Gorbsky
Dec 18, 2013·Science·Tim WangEric S Lander
Feb 22, 2014·Trends in Genetics : TIG·Joel C Eissenberg, Sarah C R Elgin
Mar 13, 2014·Trends in Cell Biology·Daniele CanzioGeeta J Narlikar

❮ Previous
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Citations

Apr 12, 2018·Critical Reviews in Biochemistry and Molecular Biology·Surinder Kumar, David B Lombard
May 17, 2019·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Yusuke OizumiJunko Kanoh
Jan 30, 2020·Cell Cycle·M Lienhard SchmitzMarkus Seibert
Feb 14, 2020·Nature Reviews. Genetics·Michael J Gutbrod, Robert A Martienssen
Aug 5, 2020·PLoS Genetics·Ujué FresánM Lluisa Espinàs
Aug 14, 2020·Cells·Silvia Meyer-NavaViviana Valadez-Graham
Oct 19, 2019·Journal of Cellular Physiology·Peiling WangJun Dai
Aug 1, 2020·Comparative Cytogenetics·Magdalena AchremAnna Kalinka
Dec 15, 2019·Genes·Colleen J Lawrimore, Kerry Bloom
Feb 6, 2020·Microbial Genomics·Gayani BatugedaraKarine G Le Roch
Jan 11, 2019·Frontiers in Genetics·Ksenia Smurova, Peter De Wulf
Feb 12, 2020·Experimental Cell Research·Jun-Ichirou OhzekiHiroshi Masumoto
Oct 17, 2020·Journal of Molecular Biology·Harsh Nagpal, Beat Fierz
May 8, 2021·Frontiers in Molecular Biosciences·Qilin LongMonika Gullerova
Dec 24, 2020·Trends in Cancer·Jessie S Kroonen, Alfred C O Vertegaal
Sep 23, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Peiling WangJun Dai

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