HPLC analysis of oleuropein, hydroxytyrosol, and tyrosol in stems and roots of Olea europaea L. cv. Picual during ripening

Journal of the Science of Food and Agriculture
Francisca Ortega-García, Juan Peragón

Abstract

Oleuropein (Ole), hydroxytyrosol (Htyr), and tyrosol (Tyr) are three of the main phenolic compounds present in the olive tree (Olea europaea L.) that have important antioxidant properties. To investigate the role of these phenolic compounds in the metabolism of stems and roots of Olea europaea L. cv. Picual during olive ripening, we identify and quantify the concentration of Htyr, Tyr, and Ole by reversed-phase high-performance liquid chromatography (RP-HPLC). Rain-fed olive trees, 30 years old, under traditional cultivation were studied in Jaén (Spain). From August to November, seven representative samples of the ripening process were taken. The concentration of these phenolic compounds proved higher in the stems than in the roots. From the middle of September to October the Htyr and Tyr concentration significantly increased in stems. The Ole concentration increased from the middle of September to the end of November. In the roots, the concentration of Htyr and Ole significantly declined during ripening. Ole, Htyr, and Tyr are present in the stems and roots of the olive tree and significantly change in concentration during ripening, demonstrating the involvement of these compounds in the metabolism of both organs during this p...Continue Reading

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Citations

Dec 18, 2013·Journal of Agricultural and Food Chemistry·Mercedes Pérez-BonillaJoaquín Altarejos
Oct 16, 2014·International Journal of Molecular Sciences·Barbara BarbaroClara Balsano
Oct 16, 2015·Critical Reviews in Food Science and Nutrition·Fátima PeresSuzana Ferreira-Dias
Jul 15, 2015·Journal of Agricultural and Food Chemistry·Sofía SalidoJoaquín Altarejos
Oct 4, 2014·Journal of Pharmaceutical and Biomedical Analysis·Pablo MirallesAmparo Salvador
Feb 24, 2015·Journal of Experimental Botany·Konstantinos KoudounasPolydefkis Hatzopoulos
May 18, 2016·Drug Metabolism Reviews·Jose Rodríguez-MoratóRafael de la Torre
Jul 8, 2016·Acta Pharmaceutica : a Quarterly Journal of Croatian Pharmaceutical Society and Slovenian Pharmaceutical Society, Dealing with All Branches of Pharmacy and Allied Sciences·Nataša ZorićIvan Kosalec
May 5, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ammad Ahmad FarooqiAnupam Bishayee
Aug 28, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Valentina Lo GiudiceLuigi Todaro
Oct 31, 2021·Journal of Food Biochemistry·Masood Sadiq ButtMuhammad Rizwan

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