HPLC Analysis, Optimization of Extraction Conditions and Biological Evaluation of Corylopsis coreana Uyeki Flos

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Ji-Hye SeoSeung-Sik Cho

Abstract

A method for the separation and quantification of three flavonoids and one isocoumarin by reverse-phase high performance liquid chromatography (HPLC) has been developed and validated. Four constituents present in a crude ethanolic extract of the flowers of Coryloposis coreana Uyeki, were analyzed. Bergenin, quercetin, quercitrin and isosalipurposide were used as calibration standards. In the present study, an excellent linearity was obtained with an r² higher than 0.999. The chromatographic peaks showed good resolution. In combination with other validation data, including precision, specificity, and accuracy, this method demonstrated good reliability and sensitivity, and can be conveniently used for the quantification of bergenin, quercetin, quercitrin and isosalipurposide in the crude ethanolic extract of C. coreana Uyeki flos. Furthermore, the plant extracts were analyzed with HPLC to determine the four constituents and compositional differences in the extracts obtained under different extraction conditions. Several extracts of them which was dependent on the ethanol percentage of solvent were also analyzed for their antimicrobial and antioxidant activities. One hundred % ethanolic extract from C. coreana Uyeki flos showed th...Continue Reading

References

Nov 1, 1995·Baillière's Clinical Rheumatology·M KloppenburgF C Breedveld
Oct 29, 1999·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·P C Hollman, M B Katan
Oct 17, 2001·Toxicon : Official Journal of the International Society on Toxinology·Solomon Habtemariam
Apr 17, 2007·Joint, Bone, Spine : Revue Du Rhumatisme·Silke SchelenzDavid Goldsmith
Oct 26, 2007·Journal of Clinical Microbiology·Bogdan Luca-HarariAftab Jasir
Apr 12, 2008·Journal of Natural Medicines·Toru YaguraHoyoku Nishino
Jun 7, 2008·Phytochemistry·Vijai K AgnihotriLarry A Walker
Jul 18, 2008·Organic & Biomolecular Chemistry·Heitor A De AbreuAntônio Flávio de C Alcântara
Sep 2, 2008·International Immunopharmacology·Jue-Hee LeeYongmoon Han
Feb 3, 2009·Cell Biochemistry and Biophysics·Nathan R Perron, Julia L Brumaghim
Mar 9, 2010·Lancet·Catherine J MathewsGerald Coakley
Apr 2, 2010·Phytochemistry·Christian GoschKarl Stich
Mar 30, 2011·Advances in Experimental Medicine and Biology·Mei ZhangPaul Okunieff
Mar 23, 2012·Bioorganic & Medicinal Chemistry Letters·Muhammad Raza ShahInam Ullah Khan
Jul 4, 2012·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Sylwia BorskaPiotr Dziegiel
Apr 3, 2013·Toxicology and Applied Pharmacology·Yuanqin YinZhuo Zhang
Apr 26, 2013·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Manh Heun KimMin Won Lee
May 21, 2013·Journal of Ethnopharmacology·Ilaine T S GehrkeAdemir F Morel
Jul 25, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Young-Hyun KimOk-Hwan Lee
Nov 29, 2015·Asian Pacific Journal of Tropical Medicine·Luís R Silva, Rafaela Teixeira

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Citations

Mar 14, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Hyung-Jae ChoiSeung-Sik Cho
Sep 8, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Seung-Hui SongSeung-Sik Cho
Aug 2, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Hyun-Jae JangMun-Chual Rho
Oct 4, 2016·The Journal of Pharmacy and Pharmacology·In-Soo YoonSeung-Sik Cho
Jul 22, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Mi-Hyeon KimSeung-Sik Cho
Dec 29, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Seung-Yub SongSeung-Sik Cho
Apr 26, 2018·Evidence-based Complementary and Alternative Medicine : ECAM·Yongjin LeeYoung-Jin Son
Feb 17, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jung-Hyun ShimSeung-Sik Cho

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