Abstract
A method for the separation and quantification of three flavonoids and one isocoumarin by reverse-phase high performance liquid chromatography (HPLC) has been developed and validated. Four constituents present in a crude ethanolic extract of the flowers of Coryloposis coreana Uyeki, were analyzed. Bergenin, quercetin, quercitrin and isosalipurposide were used as calibration standards. In the present study, an excellent linearity was obtained with an r² higher than 0.999. The chromatographic peaks showed good resolution. In combination with other validation data, including precision, specificity, and accuracy, this method demonstrated good reliability and sensitivity, and can be conveniently used for the quantification of bergenin, quercetin, quercitrin and isosalipurposide in the crude ethanolic extract of C. coreana Uyeki flos. Furthermore, the plant extracts were analyzed with HPLC to determine the four constituents and compositional differences in the extracts obtained under different extraction conditions. Several extracts of them which was dependent on the ethanol percentage of solvent were also analyzed for their antimicrobial and antioxidant activities. One hundred % ethanolic extract from C. coreana Uyeki flos showed th...Continue Reading
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Citations
Mar 14, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Hyung-Jae ChoiSeung-Sik Cho
Sep 8, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Seung-Hui SongSeung-Sik Cho
Aug 2, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Hyun-Jae JangMun-Chual Rho
Mar 1, 2019·Food Science and Biotechnology·Chun-Sik BaeTaeho Ahn
Oct 4, 2016·The Journal of Pharmacy and Pharmacology·In-Soo YoonSeung-Sik Cho
Jul 22, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Mi-Hyeon KimSeung-Sik Cho
Dec 29, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Seung-Yub SongSeung-Sik Cho
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May 11, 2017·International Journal of Molecular Medicine·In-Soo YoonSeung-Sik Cho
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Aug 29, 2021·Plants·Hyun-Woo KimWon-Keun Oh