HPLC and anti-inflammatory studies of the flavonoid rich chloroform extract fraction of Orthosiphon stamineus leaves.

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Mun Fei YamMohd Zaini Asmawi

Abstract

The aim of the present study was to verify the anti-inflammatory activity of Orthosiphon stamineus leaf extracts and to identify the active compound(s) contributing to its anti-inflammatory activity using a developed HPLC method. Active chloroform extract of O. stamineus was fractionated into three fractions using a dry flash column chromatography method. These three fractions were investigated for anti-peritoneal capillary permeability, in vitro nitric oxide scavenging activity, anti-inflammatory and nitric oxide (NO) inhibition using carrageenan-induced hind paw edema method. The flavonoid rich chloroform extract fraction (CF2) [containing sinensetin (2.86% w/w), eupatorin (5.05% w/w) and 3'-hydroxy-5,6,7,4'-tetramethoxyflavone (1.101% w/w)], significantly reduced rat hind paw edema, NO and decreased dye leakage to peritoneal cavity at p < 0.05. IC(50) of in vitro NO scavenging of CF2 was 0.3 mg/mL. These results suggest that the anti-inflammatory properties of these CF2 may possibly be due to the presence of flavonoid compounds capable of affecting the NO pathway.

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Citations

Aug 1, 2012·Journal of Medicinal Food·Omar Z AmeerMun Fei Yam
Dec 1, 2010·International Journal of Nanomedicine·Muthanna F AbdulkarimAzmin M Noor
Jul 7, 2011·International Journal of Molecular Sciences·Gedson Rodrigues de Morais LimaLeônia Maria Batista
Aug 20, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Eva ArnoldMichael Wink
Apr 13, 2011·Bioorganic & Medicinal Chemistry·Vasilis P AndroutsopoulosDemetrios A Spandidos
Mar 13, 2018·Frontiers in Pharmacology·Brandon Kar Meng ChooMohd Farooq Shaikh
Aug 25, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Zili GuoYuanyuan Xie
Jan 8, 2020·The Journal of Pharmacy and Pharmacology·Wen-Hao XuWei-Ke Su
Nov 25, 2016·BMC Complementary and Alternative Medicine·Yasser M TabanaAmin Malik Shah Abdul Majid
Jul 26, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Luya LiLantong Zhang
Jul 23, 2021·Archives of Microbiology·Abhay Prakash MishraHenrique D M Coutinho

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