HPLC and GC/MS determination of 4-aminobiphenyl haemoglobin adducts in fetuses exposed to the tobacco smoke carcinogen in utero

Toxicology
M T Pinorini-Godly, S R Myers

Abstract

Maternal-fetal exchange of the potent tobacco-related human carcinogen, 4-aminobiphenyl, was studied in women nonsmokers and in women smokers as well as in the corresponding fetuses during pregnancy. Smoking status of the women in the study was assessed via questionnaire and measurement by immunoassay of serum cotinine in maternal and fetal blood samples. 4-Aminobiphenyl was extracted from both maternal and fetal blood samples using organic solvent extractions and the released amine was qualitatively and quantitatively characterized by analysis of the samples by high pressure liquid chromatography (HPLC) and gas chromatography coupled with mass spectrometry (GC/MS). Background levels (pg 4-aminobiphenyl/g haemoglobin) of 4-aminobiphenyl-haemoglobin adducts were detected in maternal smokers (mean +/- S.D; 29.6 +/- 16.2 (GC/MS); 23.7 +/- 13.5 (HPLC) and in fetal samples (14.0 +/- 6.5 (GCMS); 10.0 +/- 4.6 (HPLC). Elevated levels of 4-aminobiphenyl-haemoglobin adducts were found in maternal smokers (488 +/- 174 (GC/MS); 423 +/- 154 (HPLC). as well as in the corresponding fetal blood samples (244 +/- 91 (GC/MS); 197 +/- 77 (HPLC). This study confirms that a potent tobacco-related carcinogen, 4-aminobiphenyl, crosses the human placen...Continue Reading

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Citations

Oct 12, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Elmar Richter, Birgit Branner
Mar 18, 2003·Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering·Hui-Ming ChenXiao-Bai Xu
Mar 10, 1999·Journal of the National Cancer Institute·G M LackmannS S Hecht
Jan 22, 2011·Birth Defects Research. Part A, Clinical and Molecular Teratology·Amy SommerJacqueline T Hecht
Feb 28, 2003·Chemico-biological Interactions·Charlene A McQueenMartin A Philbert
Apr 3, 2008·Birth Defects Research. Part C, Embryo Today : Reviews·John M Rogers
Aug 30, 2007·American Journal of Medical Genetics. Part a·Jacqueline T HechtSusan H Blanton
Nov 20, 2002·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·Gabriele Sabbioni, Christopher R Jones
Apr 23, 2014·Journal of Toxicology and Environmental Health. Part B, Critical Reviews·L L AylwardR A Becker
Apr 24, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Alberto IzzottiSilvio De Flora
Sep 22, 2017·Chemical Research in Toxicology·Gabriele Sabbioni

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