HPLC assays to simultaneously determine the angiotensin-AT1 antagonist losartan as well as its main and active metabolite EXP 3174 in biological material of humans and rats

Journal of Pharmaceutical and Biomedical Analysis
A SoldnerE Mutschler

Abstract

Novel rapid and sensitive HPLC assays were developed to simultaneously determine losartan and its main active metabolite EXP 3174 in biological material of humans and rats following solid-phase or liquid-liquid extraction. The analytes were separated on a 3 microm particle-sized ULTREMEX CN column, which was preceded by a 5 microm particle-sized guard column, using UV-detection at 245 nm. The assays provided high sensitivity with limits of quantification (LoQ) of 5 ng ml(-1) for both compounds in human and rat plasma and 10 ng ml(-1) in human and rat urine, respectively. In rat blood, bile and various tissues, limits of quantifications were achieved that ranged 10-15 ng per ml and per 100 mg tissue, respectively, for both analytes.

Citations

Jul 18, 2000·Journal of Pharmaceutical and Biomedical Analysis·G CarlucciM G Quaglia
Feb 12, 2002·Journal of Pharmaceutical and Biomedical Analysis·Anandkumari H Prabhakar, Rajani Giridhar
Sep 16, 2003·Journal of Pharmaceutical and Biomedical Analysis·Olga C LastraRenato F Pérez
Aug 30, 2003·Journal of Pharmaceutical and Biomedical Analysis·Michelle PolinkoMan-Wai Lo
Mar 7, 2000·Journal of Pharmaceutical and Biomedical Analysis·Z ZhaoD Ip
Jun 15, 2011·Acta Pharmacologica Sinica·Si-hyung YangJun-shik Choi
Jun 3, 2011·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Hye Won ParkMichael Mauer
Jul 27, 2005·Journal of Cardiovascular Pharmacology·Luis Lopez MartinezZuleica Bruno Fortes
Nov 11, 2008·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Ali Asghar Ensafi, Reza Hajian
Jul 21, 2005·Clinical Pharmacokinetics·Domenic A SicaSiddhartha Ghosh
Jun 29, 2010·Indian Journal of Pharmaceutical Sciences·K Srinivasa Rao, K Srinivas
Nov 15, 2013·Journal of Pharmaceutical Sciences·Izabela MuszalskaAnna Jelińska
Mar 8, 2016·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Mir Ali FarajzadehAzar Pazhohan
Jun 4, 2014·Luminescence : the Journal of Biological and Chemical Luminescence·Fatma Demirkaya-MilogluYucel Kadioglu
Oct 2, 2007·Journal of the Renin-angiotensin-aldosterone System : JRAAS·Hye Won ParkMichael Mauer
Sep 15, 2009·Journal of Separation Science·Hiten J ShahChhagan N Patel
May 30, 2003·Journal of Medicinal Chemistry·Boris Schmidt, Bernhard Schieffer

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