HPLC determination of phenolics adsorbed on yeasts

Journal of Pharmaceutical and Biomedical Analysis
M RizzoA Caridi

Abstract

An analytical HPLC method was developed to determine monomeric anthocyans as catechin (CA), flavonols as rutin (RU) and phenol acids as gallic acid (GA) adsorbed on the cell wall of 23 Saccharomyces cerevisiae strains grown on two media containing high levels of phenolic compounds, from grape seeds and grape skins, and on one control medium. Microbial biomass purification by liquid-liquid extraction of the phenolics is followed by reversed-phase chromatographic separation and CA, RU and GA detection by ultraviolet detector. The method was linear over the studied range of concentrations: GA at 0.12-0.96 microg/ml, CA at 0.25-20.00 microg/ml and RU at 0.02-0.20 microg/ml. The correlation coefficient for each analyte was greater than 0.9983. The recovery was greater than 85% for both GA and RU, and greater than 94% for CA. The detection limits for GA, CA and RU were determined to be 0.015, 0.025 and 0.029 microg/mg of biomass, respectively. The proposed method is highly responsive for the determination of different phenolics, and seems to be useful to evaluate their adsorption profile on yeasts.

References

Nov 23, 2000·Journal of Agricultural and Food Chemistry·S KaragiannisP Lanaridis
Mar 17, 2005·Journal of Agricultural and Food Chemistry·Giovanni SpagnaFerdinando Branca

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Citations

Jan 5, 2012·Critical Reviews in Food Science and Nutrition·C Le Bourvellec, C M G C Renard
May 1, 2008·Bioresource Technology·Luanda M A S de CamposSandra R S Ferreira
Jun 26, 2020·World Journal of Microbiology & Biotechnology·Gabriela Vollet MarsonMiriam Dupas Hubinger
Jan 13, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Dimitris SarrisSeraphim Papanikolaou

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