PMID: 6970498Oct 1, 1980Paper

HPLC-determination of some antiinflammatory, weak analgesic and uricosuric drugs in human blood plasma and its application to pharmacokinetics

Acta Pharmacologica Et Toxicologica
F Nielsen-Kudsk

Abstract

HPLC-determination of naproxen, indomethacin, ketoprofen, fenoprofen ibuprofen, diclofenac sodium, tolfenamic acid, phenylbutazone, mofebutazone, salicylic acid, acetylsalicylic acid, phenacetin, paracetamol, sulfinpyrazone and probenecid by means of an adjustable, rapid accurate and specific method is described. Plasma samples of 0.2 ml were deproteinised and the drugs extracted simultaneously with pure acetonitrile. Aliquots of 25 mul of this primary extract were directly injected on the column. As elution solvent for drug screening was basically used 55% methanol in 50 mM phosphate buffer at pH 4.0. Optimal separation of some drugs or reasonable elution times for others were obtained by varying the methanol concentration of the elution solvent or possibly its pH. The method used for individual drug determinations is very applicable for therapeutic monitoring purposes as well as for use in pharmacokinetic investigations. As an example, the practical detection limit for naproxen in plasma was about 0.2 microgram ml-1. By concentrating the extract this could be lowered to about 0.04 microgram ml-1. The method was applied in a study of the pharmacokinetics of naproxen in a person, who ingested a single oral dose of 2.5 mg kg-1. ...Continue Reading

References

Jul 1, 1976·Journal of Pharmaceutical Sciences·A J Sedman, J G Wagner
Sep 1, 1978·Clinical Pharmacokinetics·J Aarbakke
Jan 1, 1978·Australian and New Zealand Journal of Medicine·G D Champion, G G Graham
Apr 1, 1979·Journal of Pharmaceutical Sciences·R Farinotti, G Mahuzier
Feb 11, 1979·Journal of Chromatography·D Pitrè, M Grandi
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Jul 1, 1978·Journal of Pharmaceutical Sciences·K B Alton, J E Patrick
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Jun 1, 1976·Journal of Pharmacokinetics and Biopharmaceutics·K C KwanD E Duggan
Sep 1, 1975·Clinical Pharmacology and Therapeutics·G AlvánL Palmér

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Citations

Sep 1, 1992·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·E S HansenC Bünger
Feb 1, 1985·Acta Pharmacologica Et Toxicologica·J Askholt, F Nielsen-Kudsk
Aug 8, 2007·Journal of Zoo and Wildlife Medicine : Official Publication of the American Association of Zoo Veterinarians·Ursula Bechert, J Mark Christensen
Jul 1, 2011·Pharmaceutical Methods·Deepak Kumar JainNilesh Jain
Jan 1, 1985·Journal of Pharmaceutical and Biomedical Analysis·P Miskolczi, L Vereczkey

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