Hplc estimation of coenzyme Q(10) redox status in plasma after intravenous coenzyme Q(10) administration

Biomedit︠s︡inskai︠a︡ khimii︠a︡
E I KalenikovaO S Medvedev

Abstract

The pharmacokinetics of the total pool of coenzyme Q(10) (Co(10)), its oxidized (ubiquinone) and reduced (ubiquinol, CoQ(10)H₂) forms have been investigated in rats plasma during 48 h after a single intravenous injection of a solution of solubilized CoQ(10) (10 mg/kg) to rats. Plasma levels of CoQ(10) were determined by HPLC with spectrophotometric and coulometric detection. In plasma samples taken during the first minutes after the CoQ(10) intravenous injection, the total pool of coenzyme Q(10) and proportion of CoQ(10)H₂ remained unchanged during two weeks of storage at -20°C. The kinetic curve of the total pool of coenzyme Q(10) corresponds to a one-part model (R² = 0.9932), while the corresponding curve of its oxidized form fits to the two-part model. During the first minutes after the injection a significant portion of plasma ubiquinone undergoes reduction, and after 7 h the concentration of ubiquinol predominates. The decrease in the total plasma coenzyme Q(10) content was accompanied by the gradual increase in plasma ubiquinol, which represented about 90% of total plasma CoQ(10) by the end of the first day. The results of this study demonstrate the ability of the organism to transform high concentrations of the oxidized ...Continue Reading

References

Jun 1, 1992·Archives of Biochemistry and Biophysics·F AbergL Ernster
Jan 5, 2002·Journal of the American College of Nutrition·F L Crane
Jul 1, 1957·Biochimica Et Biophysica Acta·F L CRANEC WIDMER
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Apr 24, 2007·Biochemistry. Biokhimii︠a︡·E I KalenikovaO S Medvedev
Oct 5, 2007·Molecular Biotechnology·Gian Paolo Littarru, Luca Tiano
Jan 17, 2012·Journal of Bioenergetics and Biomembranes·Julie BelliereEric Fontaine

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