HPLC-FLD and spectrofluorometer apparatus: How to best detect fluorescent probe-loaded niosomes in biological samples

Colloids and Surfaces. B, Biointerfaces
Rosita PrimaveraL Di Marzio

Abstract

The analytical tools allow the detection of bioactive compounds, diagnostic agents and chemotherapeutics. Recently, new methods have been developed to analyze pharmaceutical samples and ingredients. In this attempt, analytical parameters, e.g., the lack of trueness, robustness and sensitivity, play a pivotal role to quantify and analyze molecules, both for diagnostic applications as well as therapeutic treatments. Spectrophotometers and spectrofluorometers are apparatus for easy and rapid quantification of molecular probes and chemotherapeutics into cells, plasma and tissues. However, they lack accuracy and precision. Conversely, HPLC provides the maximum resolution to detect and separate fluorescent probes and chemotherapeutics after their incubation in cells, plasma and tissues. The aim of this work was to develop an HPLC method that easily detects molecular and fluorescent probes, e.g., Nile Red, in biological samples. To improve the robustness of the method, Nile Red was analyzed before and after loading into niosomes made from Tween 20 and 21, respectively. A significant difference was further obtained by comparing the entrapment efficacy percentage of niosomes made from Tween 21 (42.23%) and Tween 20 (53.25%). The compari...Continue Reading

References

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Citations

Jan 16, 2020·Therapeutic Delivery·Swati MittalAnkit Kumar
Apr 29, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Maria Chiara CristianoDonatella Paolino

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