PMID: 9176026May 1, 1997Paper

HPLC glycosaminoglycan analysis in patients with Graves' disease

Clinical Science
C HansenG Kahaly

Abstract

1. Orbital accumulation of hydrophilic, interstitial glycosaminoglycans (GAGs) and subsequent expansion of retrobulbar tissue lead to the clinical manifestation of exophthalmos in patients with Graves' eye disease. 2. A highly specific method to determine the concentration and biochemical composition of different GAGs was developed in order to obtain a sensitive test system for the activity of the disease. By means of this method, GAG excretion in 24 h urine collections of 56 patients and 21 controls was analysed by precipitation with cetylpyridinium chloride and potassium acetate in ethanol, followed by sequential enzymic hydrolysis with chondroitin AC lyase, chondroitin ABC lyase and hyaluronate lyase, with HPLC analysis of the resulting alpha, beta-unsaturated disaccharides by anion-exchange chromatography. 3. Concentrations of GAG, chondroitin sulphate A (CA), dermatan sulphate (DS) and hyaluronic acid (HA) were determined in patients and controls, with high recovery rates [72.2 +/- 5.3%, mean +/- SEM; detection limit, 4.2 micrograms/l (0.01 mumol/l)], revealing marked differences in urinary concentrations of total GAG and HA, as well as an elevation of CA in patients compared with controls. 4. Method sensitivity was 0.86 f...Continue Reading

Citations

Jan 27, 2005·Clinical Endocrinology·C B TerweeW M Wiersinga
Nov 16, 2005·Journal der Deutschen Dermatologischen Gesellschaft = Journal of the German Society of Dermatology : JDDG·Patrick TerheydenEva B Bröcker
May 10, 2006·Clinical Chemistry and Laboratory Medicine : CCLM·Katarzyna Komosińska-VassevEwa M Koźma
Mar 6, 2002·Clinical and Experimental Immunology·M Ludgate, G Baker
Jun 30, 2010·Nature Reviews. Endocrinology·Bernadette Biondi, George J Kahaly
May 18, 2018·Ophthalmic Plastic and Reconstructive Surgery·Tanja Diana, George J Kahaly
Jan 13, 2001·Cancer Biotherapy & Radiopharmaceuticals·G J FörsterG J Kahaly
Apr 16, 2002·Thyroid : Official Journal of the American Thyroid Association·Marian E Ludgate
Oct 3, 2000·Thyroid : Official Journal of the American Thyroid Association·B RapoportS M McLachlan
Jul 21, 1998·Thyroid : Official Journal of the American Thyroid Association·G J Kahaly, G J Förster
Jan 6, 2005·Endocrine Reviews·George J Kahaly, Wolfgang H Dillmann
Mar 9, 2018·International Ophthalmology Clinics·Yao WangPradeep Mettu
Jan 14, 2000·The Journal of Clinical Endocrinology and Metabolism·G J KahalyG Hommel
Aug 5, 2020·Journal of Endocrinological Investigation·T DianaG J Kahaly
Jul 15, 1998·Medizinische Klinik·G Förster, G Kahaly

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