PMID: 3747516Jul 1, 1986Paper

HPLC isolation and GC-MS characterization of a compound strongly cross reacting with tetrahydroaldosterone antiserum

Journal of Steroid Biochemistry
T FisherD N Kirk

Abstract

Urines from patients with hypertension and elevated aldosterone levels, i.e. primary aldosteronism due to adrenal adenoma or hyperplasia or carcinoma were extracted, paper chromatographed and thereafter chromatographed repeatedly with normal phase and repeatedly with reversed phase HPLC systems in an attempt to find new metabolites of aldosterone. Specific 3 alpha-hydroxy-5 beta-tetrahydroaldosterone antiserum was used in a radioimmunoassay system to detect possible aldosterone metabolites in the HPLC fractions after each isolation step. The immunoactive HPLC fractions were derivatized and analysed by GC-MS. A relatively nonpolar compound, 11 beta:18(S),18:20 alpha-diepoxy-5 beta-pregnan-3 alpha-ol, was isolated and identified in this manner. This material was originally described by Kelly et al., in 1962 after loading human subjects with huge amounts (25-160 mg) of exogenous aldosterone. This material has not yet been described from endogenously produced aldosterone. Very small amounts, if any, were similarly isolated from the urine of a control subject. Therefore, this compound could prove to be a new marker for hypertension due to hyper-production of aldosterone.

References

Mar 1, 1978·Acta Endocrinologica·K H KohlS Abdelhamid
Jan 1, 1967·The Journal of Organic Chemistry·H Lee, M E Wolff
Oct 1, 1981·Journal of Steroid Biochemistry·K N WynneJ W Funder
Nov 1, 1984·Journal of Steroid Biochemistry·J WinterV D Bokkenheuser
Jul 1, 1983·Journal of Steroid Biochemistry·P VecseiS Lewicka
Feb 1, 1981·The Journal of Clinical Endocrinology and Metabolism·C E Gomez-Sanchez, O B Holland
Jun 1, 1963·Analytical Biochemistry·M MAKITA, W W WELLS

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Citations

Mar 1, 1988·Journal of Steroid Biochemistry·S LewickaV D Bokkenheuser
Sep 1, 1994·The Journal of Steroid Biochemistry and Molecular Biology·S AbdelhamidP Vecsei

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