HPLC method for simultaneous quantitative detection of quercetin and curcuminoids in traditional chinese medicines

Journal of Pharmacopuncture
Lee Fung AngYusrida Darwin

Abstract

Quercetin and curcuminoids are important bioactive compounds found in many herbs. Previously reported high performance liquid chromatography ultraviolet (HPLC-UV) methods for the detection of quercetin and curcuminoids have several disadvantages, including unsatisfactory separation times and lack of validation according the standard guidelines of the International Conference on Harmonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use. A rapid, specific, reversed phase, HPLC-UV method with an isocratic elution of acetonitrile and 2% v/v acetic acid (40% : 60% v/v) (pH 2.6) at a flow rate of 1.3 mL/minutes, a column temperature of 35°C, and ultraviolet (UV) detection at 370 nm was developed. The method was validated and applied to the quantification of different types of market available Chinese medicine extracts, pills and tablets. The method allowed simultaneous determination of quercetin, bisdemethoxycurcumin, demethoxycurcumin and curcumin in the concentration ranges of 0.00488 ─ 200 μg/mL, 0.625 ─ 320 μg/mL, 0.07813 ─ 320 μg/mL and 0.03906 ─ 320 μg/mL, respectively. The limits of detection and quantification, respectively, were 0.00488 and 0.03906 μg/mL for quercetin, 0.62500 and 2.50000 μg/m...Continue Reading

References

Jul 20, 1995·Cancer Letters·A J RubyR Kuttan
Jun 13, 2002·Journal of Agricultural and Food Chemistry·Guddadarangavvanahally K JayaprakashaKunnumpurath K Sakariah
Jul 31, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Kazuo IshiiYasuji Kasuya
Aug 21, 2003·Journal of Agricultural and Food Chemistry·Maria CareriIngrid Zagnoni
Jun 14, 2006·Molecular and Cellular Biochemistry·Manikandan PanchatcharamLonchin Suguna
Aug 19, 2007·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·M HarwoodT C Lines
Dec 9, 2008·International Immunopharmacology·Hee-ju ParkYeong-Min Park
Feb 4, 2009·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·S MahattanadulP Bouking
Feb 27, 2009·Pathology, Research and Practice·Bao-Feng HuangXin Wang
Mar 12, 2009·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Junwei ZhangKenichiro Nakashima
Apr 30, 2009·Phytochemical Analysis : PCA·Wisut WichitnithadPornchai Rojsitthisak
Aug 12, 2009·Biochemical and Biophysical Research Communications·Teayoun KimSuresh T Mathews
Sep 25, 2009·International Journal of Food Microbiology·Yu WangFengxia Lv
Aug 7, 2010·Colloids and Surfaces. B, Biointerfaces·Aditya P NayakEliana B Souto
Feb 17, 2011·Journal of Agricultural and Food Chemistry· BhawanaNidhi Jain
Feb 15, 2012·Advances in Nutrition·Abigail J LarsonThunder Jalili
Jan 2, 2013·Taiwanese Journal of Obstetrics & Gynecology·Oliver B VillafloresTzong-Yuan Wu
Jan 30, 2013·Daru : Journal of Faculty of Pharmacy, Tehran University of Medical Sciences·Mahendra K VermaRakesh K Johri
Aug 3, 2013·The Brazilian Journal of Infectious Diseases : an Official Publication of the Brazilian Society of Infectious Diseases·Kunal JainK Gowthamarajan

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Citations

Aug 25, 2016·Biomedical Chromatography : BMC·Khaled S AbdelkawyFawzy Elbarbry
May 15, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Amrit PoudelHyeong-Kyu Lee
Jan 2, 2019·Evidence-based Complementary and Alternative Medicine : ECAM·Stephanie Triseptya HuntoJae Youl Cho

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Methods Mentioned

BETA
column chromatography

Software Mentioned

SPSS

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