PMID: 9530962Apr 8, 1998Paper

HPLC method for the determination of Fuc to Asn-linked GlcNAc fucosyltransferases

Glycoconjugate Journal
A RoitingerFriedrich Altmann

Abstract

Mammalian cells often contain an enzyme which transfers fucose onto the reducing terminal GlcNAc (GlcNAc-1) of N-glycans with an alpha1,6-linkage. In plants, on the other hand, the fucose is transferred to GlcNAc-1 with an alpha1,3-inkage. Insect cells can exhibit both enzymatic activities. Hitherto, the activity of these fucosyltransferases has been determined by the incorporation of radioactively labelled fucose into an acceptor glycopeptide. This assay, however, cannot discriminate these two activities. Here we report on the use of dansylated glycoasparagine for the specific determination of 1,3- and 1,6-fucosyltransferases. The two possible products and the substrate are separated on a reversed phase column and detected by fluorescence.

Citations

Dec 21, 2010·The Journal of Biological Chemistry·Josephine GrassFriedrich Altmann
Dec 18, 2004·Glycobiology·Katharina PaschingerIain B H Wilson
Sep 15, 2004·The Journal of Biological Chemistry·Katharina PaschingerIain B H Wilson
Nov 27, 2016·Analytical and Bioanalytical Chemistry·Gerda C M Vreeker, Manfred Wuhrer
Aug 20, 2003·Accounts of Chemical Research·Noboru TomiyaYuan C Lee
Dec 2, 1999·Biochimica Et Biophysica Acta·E StaudacherL März
May 29, 2003·Biochimie·Monika BencúrováIain B H Wilson

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