HPLC-MS analysis of iodotyrosines produced by sample hydrolysis: A simple method for monitoring iodinated casein in feed premixes

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Zongyi WangWenjun Yang

Abstract

A new and simple HPLC-MS method was developed for monitoring iodinated casein in feed premixes. In this method, feed premixes were hydrolyzed, and the iodotyrosines thus released were analyzed. Sample pretreatment included precipitation of transition metals ions with Na(2)S, hydrolysis with sodium hydroxide, and cleaning up with an Oasis SAX cartridge. Gradient elution was carried out on a C(18) column with water (containing 0.1% formic acid) and acetonitrile. Ion detection was performed using ESI positive SIM at m/z 262, 308, 388, and 434. Iodinated casein levels were monitored by qualitative analysis of the iodotyrosines released upon sample hydrolysis and by quantifying the 3,5-diiodotyrosine released. The validation data demonstrated that the method was selective and sensitive (<or=0.2mgg(-1)) for iodinated casein and had acceptable accuracy (recoveries: 81.3-106.7%) and precision (RSD: 1.7-16.0%).

References

Oct 3, 1986·Journal of Chromatography·T J CarneL A Edwards
Oct 1, 1971·Analytical Biochemistry·N N NiheiC S Hollander
Feb 1, 1970·Journal of Dairy Science·T W Mischler, E P Reineke
Dec 31, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Helen GikaWolfgang Lindner
Dec 21, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Helen G GikaIoannis N Papadoyannis

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