HPLC quantification of doxorubicin in plasma and tissues of rats treated with doxorubicin loaded poly(alkylcyanoacrylate) nanoparticles

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Khairallah AlharethFathi Moussa

Abstract

The long-term clinical use of doxorubicin (Dox), one of the most important anticancer agent in use, is limited by dose-related acute cardiotoxicity, myelo-suppression and multidrug resistance developed by cancer cells. To improve the antitumor efficacy and reduce the toxicity of Dox, many drug delivery systems have been developed, including poly(alkylcyanoacrylate) (PACA) nanoparticles. A new formulation of PACA nanoparticles with potential stealth properties were prepared by redox radical emulsion polymerization and associated to Dox in our laboratory. To comparatively investigate the pharmacokinetics and the biodistribution of different formulations of Dox associated PACA nanoparticles, a simple and rapid high performance liquid chromatographic method (HPLC) was developed for the quantification of Dox in plasma and tissues of rats treated with Dox loaded PACA nanoparticle (Dox-PACA). Dox was eluted at 4.4 min and it was well separated from its main metabolites doxorubicinol (Doxl) and doxorubicinon (Doxon) and idarubicin (Ida) used as internal standard (IS). Extraction of Dox from biological media was achieved by liquid-liquid extraction. The recovery of total Dox (i.e. free Dox and Dox associated with nanoparticles) from pla...Continue Reading

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Citations

Jun 9, 2014·Materials Science & Engineering. C, Materials for Biological Applications·Betiana FeliceSeeram Ramakrishna
Aug 8, 2014·Journal of Pharmaceutical Sciences·Caroline Damico CandidoRosângela Gonçalves Peccinini
Apr 3, 2012·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Khairallah AlharethFathi Moussa
Mar 15, 2016·Nature Biotechnology·Rong XuHaifa Shen
Jun 1, 2017·The Analyst·Nicolas GuichardSandrine Fleury-Souverain
Jul 29, 2017·Chemical Reviews·Zdeněk FarkaPetr Skládal
Feb 21, 2018·Artificial Cells, Nanomedicine, and Biotechnology·Güliz AkSenay Hamarat Sanlier

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