HPLC retention time as a diagnostic tool for hemoglobin variants and hemoglobinopathies: a study of 60000 samples in a clinical diagnostic laboratory

Clinical Chemistry
Alla JoutovskyMichael A Nardi

Abstract

Previous evaluations of HPLC as a tool for detection of hemoglobin variants have done so within newborn-screening programs and/or by use of stored samples. We describe a 32-month prospective study in a clinical diagnostic laboratory in which we evaluated the imprecision of HPLC retention times and determined the retention times for hemoglobin variants seen in a multiethnic setting. We analyzed all samples on the Bio-Rad Variant II HPLC system. For normal hemoglobin fractions and hemoglobin variants, we recorded and analyzed their retention times, their proportion of the total hemoglobin (%), and the peak characteristics. We compared the imprecision of retention time with the imprecision of retention time normalized to the retention time of hemoglobin A0 (Hb A0) and to the retention time of Hb A2. Alkaline and acid hemoglobin electrophoresis, and in certain cases globin chain electrophoresis, isoelectric focusing, and DNA analysis, were performed to document the identities of the hemoglobin variants. The mean (SD) imprecision (CV) of the retention time was 1.0 (0.7)% with no statistical difference compared with the imprecision for normalized retention times. Among 60,293 samples tested, we encountered 34 unique hemoglobin varian...Continue Reading

References

Mar 4, 1967·Nature·H M RanneyR L Nagel
Nov 14, 1997·Clinical and Laboratory Haematology·B J Wild, A D Stephens
Jul 23, 1998·British Journal of Haematology
Nov 6, 2001·Clinica Chimica Acta; International Journal of Clinical Chemistry·C N Ou, C L Rognerud
Nov 29, 2002·Archives of Pathology & Laboratory Medicine·John D LaffertyDavid H K Chui

❮ Previous
Next ❯

Citations

Jul 5, 2013·Archives of Gynecology and Obstetrics·Sheng HeChenguang Zheng
Oct 22, 2014·Indian Journal of Hematology & Blood Transfusion : an Official Journal of Indian Society of Hematology and Blood Transfusion·Aastha GuptaManorama Bhargava
Aug 14, 2013·Bioanalysis·Rebecca L EdwardsHelen J Cooper
Jan 16, 2014·Indian Journal of Hematology & Blood Transfusion : an Official Journal of Indian Society of Hematology and Blood Transfusion·Meet KumarManorama Bhargava
Oct 11, 2012·Revista brasileira de hematologia e hemoterapia·Renan Gracia de OliveiraClaudia Regina Bonini-Domingos
May 15, 2009·International Journal of Hematology·Noppacharn UaprasertPranee Sutcharitchan
Jun 11, 2010·Laboratory Hematology : Official Publication of the International Society for Laboratory Hematology·Nadja K Burns, Semyon A Risin
Aug 19, 2007·Indian Journal of Pediatrics·R B ColahA C Gorakshakar
Apr 25, 2009·Indian Journal of Pediatrics·Aruna RanganM Bhargava
Mar 11, 2011·Indian Journal of Pediatrics·Deeparaj Ganapati HegdeG P Prashanth
Apr 22, 2005·Clinical Chemistry and Laboratory Medicine : CCLM·Jean RiouJosiane Bardakdjian
Mar 8, 2006·Clinical Chemistry and Laboratory Medicine : CCLM·Amal LouahabiDiane Maisin
May 10, 2008·Annals of Hematology·Nazish Khalid HashmiMashhooda Rasool Hashmi
Mar 27, 2012·BMC Blood Disorders·Bushra MoizTariq Moatter
Jan 22, 2010·Indian Journal of Pathology & Microbiology·Ritesh SachdevGaurav Tyagi
Nov 4, 2010·Indian Journal of Pathology & Microbiology·Amit K Yadav
Jan 17, 2014·Journal of Obstetrics and Gynaecology of India·Dipal S BhukhanvalaSnehalata C Gupte
Dec 22, 2009·Clinical Chemistry and Laboratory Medicine : CCLM·Jean-Paul ChapelleWouter van den Eshof
Apr 22, 2006·American Journal of Clinical Pathology·Peter J HowanitzYeshpal S Chauhan
Sep 14, 2011·Hemoglobin·Carolyn C Hoppe
Oct 16, 2014·Clinica Chimica Acta; International Journal of Clinical Chemistry·Dina N GreeneArchana M Agarwal
Sep 22, 2012·Clinical Biochemistry·Kamran MoradkhaniHenri Wajcman
Mar 21, 2012·American Journal of Clinical Pathology·David F KerenDeborah Hedstrom
Jun 21, 2011·American Journal of Clinical Pathology·Sitthichai PanyasaiSakorn Pornprasert
Aug 22, 2007·American Journal of Clinical Pathology·Richard T ScuderiRobert L Fitzgerald
Jun 3, 2009·International Journal of Laboratory Hematology·P Van DelftP C Giordano
Feb 26, 2008·International Journal of Laboratory Hematology·D Abdel-GadirB J Bain
Dec 20, 2005·Transfusion Medicine·T N HigginsG Clarke
Jul 31, 2008·International Journal of Laboratory Hematology·P VermeerschW Goossens
Jul 5, 2007·European Journal of Haematology·John N WaitumbiBernhards R Ogutu
Mar 26, 2011·International Journal of Laboratory Hematology·M S M KhalilJ Old
Apr 5, 2012·International Journal of Laboratory Hematology·E S EdisonR V Shaji
Apr 18, 2013·International Journal of Laboratory Hematology·C C Hoppe
Dec 17, 2009·Transfusion Medicine·A K TiwariA Ahuja
Jan 25, 2013·Case Reports in Genetics·Donovan CalderJennifer M Knight-Madden
Dec 14, 2005·European Journal of Haematology·Vip ViprakasitPipat Chotimarat
Jun 25, 2015·British Journal of Haematology·Martin H Steinberg, Griffin P Rodgers
May 24, 2015·Journal of the American Society for Mass Spectrometry·Roger ThébergeMark E McComb

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.