PMID: 11909653Mar 23, 2002Paper

HPLC simultaneous determination of glycerol and mannitol in human tissues for forensic analysis

Forensic Science International : Synergy
Akiko KiyoshimaN Ikeda

Abstract

A method for simultaneous determination of glycerol and mannitol in various human tissues was devised and for this we used high-performance liquid chromatography (HPLC). Specimens were homogenized in a mixture of chloroform and methanol, phosphate buffer (pH 7.0) and pentaerythritol (IS) solution. After centrifugation, an aliquot of the aqueous layer was evaporated to dryness and derivatized with p-nitrobenzoyl chloride at 50 degrees C for 1h, then applied to HPLC with analytical conditions of: column, CAPCELL PAK C18 MG (250 mm x 3.0 mm i.d., 5 microm, Shiseido Co. Ltd., Tokyo, Japan); column temperature, 1-2 degrees C; mobile phase, 75% acetonitrile-distilled water containing 0.05% trifluoroacetic acid, 0.05% heptafluoro-n-butyric acid and 0.1% triethylamine; flow rate, 0.5 ml/min; wavelength, 260 nm. Calibration curves for both substances were linear in concentration ranges from 1 to 500 microg/0.1g and correlation coefficients exceeded 0.99. The relative standard deviation (R.S.D.) of the method was evaluated at concentrations of 10 and 100 microg/0.1g, and ranged from 0.84 to 10.6%. Using this method, we determined the regional distribution levels of glycerol and mannitol in various tissues from an autopsied brain dead man.

References

Oct 1, 1992·Journal of Neurosurgery·A M Kaufmann, E R Cardoso
Jun 25, 1966·Nature·H A SloviterK Suhara
Jan 1, 1983·Journal of Pharmaceutical Sciences·T MarunakaY Minami
Dec 16, 1994·Forensic Science International : Synergy·M Oehmichen
Jan 23, 1998·Forensic Science International : Synergy·N JitsufuchiN Ikeda
Aug 3, 2001·Journal of Chromatography. B, Biomedical Sciences and Applications·A KiyoshimaN Ikeda

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Citations

May 25, 2012·The Journal of Clinical Investigation·Layla K MahdiAbiodun D Ogunniyi
Feb 15, 2005·Legal Medicine·Akiko KiyoshimaNoriaki Ikeda
Feb 21, 2006·Journal of Pharmaceutical and Biomedical Analysis·Robert Forget, Suzanne Spagnoli
Apr 20, 2016·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Ying DongJinglin Ding
Dec 12, 2018·Journal of the Science of Food and Agriculture·Luchen RuanLihua Hou

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