HPLC study on Fenton-reaction initiated oxidation of salicylic acid. Biological relevance of the reaction in intestinal biotransformation of salicylic acid

Free Radical Research
Eszter NyúlPál Perjési

Abstract

Fenton-reaction initiated in vitro oxidation and in vivo oxidative biotransformation of salicylic acid was investigated by HPLC-UV-Vis method. By means of the developed high performance liquid chromatography (HPLC) method salicylic acid, catechol, and all the possible monohydroxylated derivatives of salicylic acid can be separated. Fenton oxidations were performed in acidic medium (pH 3.0) with two reagent molar ratios: (1) salicylic acid: iron: hydrogen peroxide 1:3:1 and (2) 1:0.3:1. The incubation samples were analysed at different time points of the reactions. The biological effect of elevated reactive oxygen species concentration on the intestinal metabolism of salicylic acid was investigated by an experimental diabetic rat model. HPLC-MS analysis of the in vitro samples revealed presence of 2,3- and 2,5-dihydroxybenzoic acids. The results give evidence for nonenzyme catalysed intestinal hydroxylation of xenobiotics.

References

Dec 1, 1992·Free Radical Biology & Medicine·A GhiselliA Ferro-Luzzi
Jan 1, 1991·Free Radical Biology & Medicine·B HalliwellM Ingelman-Sundberg
Jan 15, 1988·Biochemical Pharmacology·M Grootveld, B Halliwell
Mar 1, 1986·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·A J HuttR L Smith
Mar 12, 2003·Journal of Hazardous Materials·E Neyens, J Baeyens
Sep 9, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Eiichi YamamotoNaoki Asakawa
Mar 12, 2008·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Doreen MitschkeUrsula Moenning
Nov 11, 2008·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Budhi OktaviaToyohide Takeuchi
Mar 3, 2009·Clinical Gastroenterology and Hepatology : the Official Clinical Practice Journal of the American Gastroenterological Association·Edgardo SmecuolAngel Lanas
May 7, 2010·Free Radical Biology & Medicine·Ralf P BrandesKatrin Schröder
Jul 20, 2010·Chemico-biological Interactions·Dominique ZiechMihalis I Panayiotidis
Dec 22, 2010·Advances in Skin & Wound Care·Najat ZiyadehJohn D Seeger
Aug 1, 1995·Environmental Science & Technology·S M ArnoldR F Harris
Aug 25, 2012·American Journal of Physiology. Gastrointestinal and Liver Physiology·Akifumi FukuiToshikazu Yoshikawa
Dec 13, 2012·Acta Physiologica Hungarica·S BojcsevEmil Fischer
Aug 27, 2014·Free Radical Biology & Medicine·Ralf P BrandesKatrin Schröder
Apr 4, 2015·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Mirza BojićF Peter Guengerich
Jun 30, 2016·Biomedical Chromatography : BMC·Mónika KuzmaPál Perjési
Oct 19, 2016·Saudi Pharmaceutical Journal : SPJ : the Official Publication of the Saudi Pharmaceutical Society·Ullah AsmatKhan Ismail

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Citations

Jul 25, 2019·Canadian Journal of Physiology and Pharmacology·Noémi-Piroska KovácsPál Perjési

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