HPLC/ESI-MS and NMR analysis of flavonoids and tannins in bioactive extract from leaves of Maytenus ilicifolia

Journal of Pharmaceutical and Biomedical Analysis
Lauro M de SouzaGuilherme Lanzi Sassaki

Abstract

Maytenus ilicifolia is an important plant in Brazilian folk medicine, used in many gastric disorder treatments. Low molecular weight components present in the leaves have been characterized as afzelechin, epiafzelechin, catechin, epicatechin, gallocatechin and epigallocatechin, as detected by HPLC and ESI-MS. Condensed tannins have also been found, consisting of di-, tri-, tetra-, and penta-, hexa, and heptamers. ESI-MS analyses were performed in positive and negative ionization modes, and in contrast with other investigations, negative ionization improved sensitivity for obtaining molecular ions. Moreover, the tandem-MS profile with negative detection provided characteristic fragments, such as those found at m/z 543 [(epi)afzelechin-(epi)afzelechin], m/z 561 [(epi)afzelechin-(epi)catechin], and m/z 577 [(epi)catechin-(epi)catechin] or [(epi)afzelechin-(epi)gallocatechin]. The analysis of the fragments also indicated the presence of additional ether linkage between C2 and C7, present in A-Type proanthocyanidins, and were identified at m/z 559 [(epi)afzelechin-(epi)catechin], m/z 575 [(epi)catechin-(epi)catechin] or [(epi)afzelechin-(epi)gallocatechin] and at m/z 591, [(epi)catechin-(epi)gallocatechin]. CID-fragmentation was use...Continue Reading

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Citations

Jul 13, 2012·Journal of Agricultural and Food Chemistry·María BlanchCarmen Merodio
Oct 9, 2014·BMC Complementary and Alternative Medicine·Azila Abdul KarimNur Azilah Abdullah
Aug 3, 2011·Journal of Pharmaceutical and Biomedical Analysis·Anna BianchiSanto Scalia
Jun 18, 2014·Natural Product Research·Mohamed A FaragLudger A Wessjohann
Aug 21, 2015·Environmental Science. Processes & Impacts·Sara SandronBrett Paull
Jan 5, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Javier E RamirezMario J Simirgiotis
Mar 5, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Wan-Yi GuJian-Lin Wu
Jul 6, 2013·Experimental Biology and Medicine·Raimundo Fernandes de Araújo JúniorAurigena Antunes de Araújo
Mar 1, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Shiuh-Tsuen HuangJi-Yuan Liang
Jan 6, 2021·Antibiotics·Igor Trujillo-MayolJulio Alarcón-Enos
Jan 30, 2021·Food Research International·Junfang WangLijun Wang
Dec 19, 2020·Comprehensive Reviews in Food Science and Food Safety·Ana Sanches SilvaSeyed Mohammad Nabavi
Apr 4, 2021·Foods·Margherita LavorgnaMarina Isidori
May 1, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Fernanda das Neves CostaSvetlana Ignatova
May 1, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Lili ZhenClaudia Crestini
Aug 27, 2021·BioMed Research International·Babita AryalNiranjan Parajuli

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