HPV-16 targeted DNA vaccine expression: The role of purification

Biotechnology Progress
A M AlmeidaA Sousa

Abstract

DNA vaccines have come to light in the last decades as an alternative method to prevent many infectious diseases, but they can also be used for the treatment of specific diseases, such as cervical cancer caused by Human Papillomavirus (HPV). This virus produces E6 and E7 oncoproteins, which alter the cell cycle regulation and can interfere with the DNA repairing system. These features can ultimately lead to the progression of cervical cancer, after cell infection by HPV. Thus, the development of a DNA vaccine targeting both proteins arises as an interesting option in the treatment of this pathology. Nonetheless, before evaluating its therapeutic potential, the purity levels of a biopharmaceutical must meet the regulatory agency specifications. Previously, our research group successfully purified the supercoiled isoform of the recombinant HPV-16 E6/E7 DNA vaccine with virtual 100% purity by affinity chromatography. The present work was designed to evaluate the effect that pDNA sample purity levels may exert in the expression of a target protein. Thus, in vitro studies were performed to assess the vaccine ability to produce the target proteins and to compare the expression efficiency between the pDNA sample obtained by affinity c...Continue Reading

References

Aug 19, 2000·BioTechniques·A ColosimoD C Gruenert
Mar 14, 2007·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Zhong WeiZhi Yuan
Aug 5, 2008·Trends in Biotechnology·Fani SousaJoão A Queiroz
Oct 17, 2009·The AAPS Journal·Mohammed S Al-Dosari, Xiang Gao
Oct 15, 2010·Human Gene Therapy·Christine I WooddellJon A Wolff
Jan 17, 2012·Vaccine·Arjun DhanoyaEli Keshavarz-Moore
Sep 18, 2012·Journal of Biotechnology·Lei LiNikolai Petrovsky
Oct 30, 2012·The Journal of Infection·Carol ChelimoJ Mark Elwood
May 29, 2013·Virology·Scott B Vande Pol, Aloysius J Klingelhutz
Jun 5, 2013·Virology·Ann Roman, Karl Munger
Sep 10, 2013·Methods in Enzymology·Laura Koontz
Oct 5, 2013·Trends in Genetics : TIG·Bijoyita Roy, Allan Jacobson
Dec 30, 2014·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·A M AlmeidaA Sousa
Sep 2, 2015·Nature Reviews. Clinical Oncology·F Xavier BoschJack Cuzick
Jan 29, 2016·Scientific Reports·Patrícia A PereiraFani Sousa

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