HPV caught in the tetraspanin web?

Medical Microbiology and Immunology
Jérôme FinkeThorsten Lang

Abstract

Tetraspanins are master organizers of the cell membrane. Recent evidence suggests that tetraspanins themselves may become crowded by virus particles and that these crowds/aggregates co-internalize with the viral particles. Using microscopy, we studied human papillomavirus (HPV) type 16-dependent aggregates on the cell surface of tetraspanin overexpressing keratinocytes. We find that aggregates are (1) rich in at least two different tetraspanins, (2) three-dimensional architectures extending up to several micrometers into the cell, and (3) decorated intracellularly by filamentous actin. Moreover, in cells not overexpressing tetraspanins, we note that obscurin-like protein 1 (OBSL1), which is thought to be a cytoskeletal adaptor, associates with filamentous actin. We speculate that HPV contact with the cell membrane could trigger the formation of a large tetraspanin web. This web may couple the virus contact site to the intracellular endocytic actin machinery, possibly involving the cytoskeletal adaptor protein OBSL1. Functionally, such a tetraspanin web could serve as a virus entry platform, which is co-internalized with the virus particle.

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Citations

Jul 25, 2020·Medical Microbiology and Immunology·Luise Florin, Charlotte M de Winde
May 12, 2021·Infectious Agents and Cancer·Maria IsaguliantsFranco M Buonaguro
Jun 3, 2021·Viruses·Kaitlyn SpeckhartBilly Tsai
Nov 2, 2021·Antimicrobial Agents and Chemotherapy·Jesse M YoungMichelle A Ozbun

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Methods Mentioned

BETA
superresolution microscopy
confocal microscopy
transfection
fluorescence microscopy

Software Mentioned

ImageJ

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