HPV16-E7-Specific Activated CD8 T Cells in E7 Transgenic Skin and Skin Grafts

Frontiers in Immunology
Seyed Davoud JazayeriIan H Frazer

Abstract

Human papillomavirus (HPV) 16 E7 (E7) protein expression in skin promotes epithelial hyperproliferation and transformation to malignancy. Grafts of murine skin expressing E7 protein as a transgene in keratinocytes are not rejected from immunocompetent recipients, whereas grafts expressing ovalbumin (OVA), with or without coexpression of E7 protein, are promptly rejected, demonstrating that E7-associated non-antigen-specific local immunosuppression is not a major determinant of lack of rejection of E7 transgenic skin. To determine whether failure of rejection of E7 skin grafts is due to failure to attract E7-specific effector T cells, E7- and OVA-specific effector CD8(+) T cells, activated in vitro, were transferred to animals bearing E7 transgenic skin grafts. Three days after T cell transfer, E7-specific T cells were present in significantly greater numbers than OVA-specific T cells in the grafted skin on animals bearing recently placed or healed E7 grafts, without graft rejection, and also in the ear skin of E7 transgenic animals, without obvious pathology. E7 and OVA-specific T cells were present in lesser numbers in healed E7 grafts than in recently placed grafts and in lesser numbers in recently placed E7 transgenic epider...Continue Reading

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Citations

Oct 16, 2018·Frontiers in Medicine·Paula T KuoGraham R Leggatt
Nov 2, 2020·The Journal of Investigative Dermatology·Abate Assefa BashawJanin Chandra

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Methods Mentioned

BETA
transgenic
flow cytometry
FACS
Fluorescence Imaging

Software Mentioned

Kaluza
Prism
GraphPad

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