PMID: 3759265Oct 15, 1986Paper

HRAS1-selected, chromosome-mediated transformants vary in phenotype in vitro and tumorigenic potential in vivo

International Journal of Cancer. Journal International Du Cancer
D J PorteousC M Steel

Abstract

Transfection of mouse C127 cells with mitotic chromosomes isolated from a human EJ bladder carcinoma cell line gave rise, at high frequency, to foci of transformed cells. Independent, HRAS1-selected chromosome-mediated transformants displayed distinctive cellular morphologies in monolayer culture and colony-forming abilities in low-melting-point agarose. Subcutaneous inoculation of neonatally thymectomized, Ara-C-protected, total-body-irradiated CBA mice was used to compare the tumorigenic potential of each transformant. Significant quantitative and qualitative differences in tumorigenicity were found between transformants which correlated with differences in malignant phenotype observed in vitro. The sensitivity of the tumorigenicity assay is such that rare transformation events can be selected directly in vivo.

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Citations

May 1, 1989·Somatic Cell and Molecular Genetics·W A BickmoreD J Porteous
Jan 1, 1990·Somatic Cell and Molecular Genetics·D J PorteousV van Heyningen

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