HrpN of Erwinia amylovora functions in the translocation of DspA/E into plant cells.

Molecular Plant Pathology
Ana M BocsanczySteven V Beer

Abstract

The type III secretion system (T3SS) is required by plant pathogenic bacteria for the translocation of certain bacterial proteins to the cytoplasm of plant cells or secretion of some proteins to the apoplast. The T3SS of Erwinia amylovora, which causes fire blight of pear, apple and other rosaceous plants, secretes DspA/E, which is an indispensable pathogenicity factor. Several other proteins, including HrpN, a critical virulence factor, are also secreted by the T3SS. Using a CyaA reporter system, we demonstrated that DspA/E is translocated into the cells of Nicotiana tabacum'Xanthi'. To determine if other T3-secreted proteins are needed for translocation of DspA/E, we examined its translocation in several mutants of E. amylovora strain Ea321. DspA/E was translocated by both hrpW and hrpK mutants, although with some delay, indicating that these two proteins are dispensable in the translocation of DspA/E. Remarkably, translocation of DspA/E was essentially abolished in both hrpN and hrpJ mutants; however, secretion of DspA/E into medium was not affected in any of the mentioned mutants. In contrast to the more virulent strain Ea273, secretion of HrpN was abolished in a hrpJ mutant of strain Ea321. In addition, HrpN was weakly tra...Continue Reading

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Citations

Jun 12, 2013·Molecular Plant-microbe Interactions : MPMI·Min-Seon ChoiChang-Sik Oh
Feb 10, 2012·Molecular Plant-microbe Interactions : MPMI·Manon MoreauMathilde Fagard
May 22, 2009·Plant Physiology·Daniela Büttner, Sheng Yang He
Apr 13, 2010·Applied and Environmental Microbiology·Lisa M SchechterHari B Krishnan
Oct 26, 2013·Molecular Plant-microbe Interactions : MPMI·E VergneE Chevreau
Jun 7, 2014·Annual Review of Phytopathology·Jeff H ChangAllison L Creason
Jun 19, 2012·Annual Review of Phytopathology·Mickael MalnoyBrion Duffy
Aug 7, 2010·Annual Review of Phytopathology·David J Schneider, Alan Collmer
Apr 25, 2012·Plant Science : an International Journal of Experimental Plant Biology·Thomas Dugé De BernonvilleMarie-Noëlle Brisset
Jun 8, 2012·Molecular Plant Pathology·John MansfieldGary D Foster
Jun 1, 2013·Molecular Plant Pathology·Laura S RamosTimothy W McNellis
May 8, 2010·Molecular Plant Pathology·Jian-Gang LiJian-Hua Guo
Mar 6, 2015·The Plant Journal : for Cell and Molecular Biology·Emilie ChapelleFrédérique Van Gijsegem
Apr 12, 2014·Frontiers in Plant Science·Anastasia P Tampakaki
Oct 5, 2017·Molecular Plant Pathology·Mo WangGuo-Liang Wang
Jul 13, 2018·Molecular Plant-microbe Interactions : MPMI·Manuela CampaMickael Malnoy
Mar 19, 2020·International Journal of Molecular Sciences·Tingyan SunHuasong Zou
Sep 6, 2018·BMC Microbiology·Xuan WangHansong Dong
Jun 14, 2019·Frontiers in Plant Science·Liyuan ZhangHansong Dong
Dec 15, 2017·The Plant Pathology Journal·Gnanendra Shanmugam, Junhyun Jeon

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