HSA carbonylation with methylglyoxal and the binding/release of copper(II) ions

Metallomics : Integrated Biometal Science
Ana Z PenezićLjuba M Mandić

Abstract

The potential of carbonylation with methylglyoxal to alter HSA's binding affinity for copper(II) ions and its influence on the release of copper(II) ions from copper-HSA complexes were studied. The affinity of HSA to coordinate copper(II) decreased upon carbonylation of the Cys34-SH group. Carbonylation of copper-HSA complexes caused a decrease in Cys34-SH content, conformational changes and the release of copper(II) ions. The ratio between the percentage of reduction in the Cys34-SH group content and the percentage of release of copper(II) from complexes is 2.12 ± 0.28. Because the same ratio (1.96 ± 0.36) was obtained upon oxidation of the Cys34-SH group (with no changes in HSA conformation), the binding/release of copper (II) by HSA depended mainly on the redox state of the Cys34-SH group. The contents of Cys34-SH and HSA-bound copper(II) IONS in the diabetic group (0.457 ± 0.081 mol SH per mol HSA, 10.7 ± 0.01 mmol per mol HSA, resp.) were significantly lower (p < 0.01) compared to the control group (0.609 ± 0.027 mol SH per mol HSA; 13.4 ± 0.01 mmol per mol HSA, resp.). Very strong correlations between the values for HSA-SH and glycated haemoglobin, HbA1c, (R = -0.803, p < 0.01), and between the values for the HSA-bound co...Continue Reading

References

Feb 15, 1993·European Journal of Biochemistry·S A Phillips, P J Thornalley
Jun 24, 1998·Biochemistry·G BulajD P Goldenberg
May 26, 2001·Biochemical and Biophysical Research Communications·H ZoellnerG Lynch
Aug 14, 2001·Renal Failure·M L WrattenC Tetta
Apr 6, 2002·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Yi Zhang, Dean E Wilcox
Nov 6, 2003·Clinical Chemistry and Laboratory Medicine : CCLM·Annunziata LapollaPietro Traldi
Nov 19, 2003·Archives of Biochemistry and Biophysics·Mariana D Argirova, Beryl J Ortwerth
Dec 29, 2004·Diabetes Research and Clinical Practice·Nessar Ahmed
Jun 17, 2005·Diabetes & Metabolism·P FaureS Halimi
Jul 22, 2005·Clinical Science·Casper G Schalkwijk, Coen D A Stehouwer
Jul 23, 2005·Annals of the New York Academy of Sciences·Paul J Thornalley
Oct 25, 2006·Amino Acids·S CarballalR Radi
May 23, 2007·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Małgorzata RózgaWojciech Bal
Jan 10, 2008·Diabetes Care·UNKNOWN American Diabetes Association
Jan 15, 2008·Biological Trace Element Research·Tasneem Gul KaziGhulam Abbas Kandhro
Feb 8, 2008·The European Respiratory Journal·P FaureJ-L Pépin
May 14, 2008·FEBS Letters·Marjolaine RocheEmmanuel Bourdon
May 24, 2008·Biochimica Et Biophysica Acta·Karl OettlRudolf E Stauber
Sep 18, 2009·Journal of Pharmaceutical Sciences·Katsumi MeraMasaki Otagiri
May 14, 2010·Nature·Lucia BanciPeep Palumaa
Jul 7, 2010·Chemico-biological Interactions·María Eugenia LetelierPaula Aracena-Parks
Dec 21, 2010·Biochimie·Philippe Rondeau, Emmanuel Bourdon
Aug 13, 2011·Clinica Chimica Acta; International Journal of Clinical Chemistry·Stefan W Vetter, Venkata S K Indurthi
Feb 22, 2012·Diabetes & Metabolism·A Guerin-DubourgP Rondeau
Sep 18, 2012·Endocrine·Paulo MatafomeRaquel Seiça
Jan 1, 2010·Indian Journal of Clinical Biochemistry : IJCB·A SarkarM Prakash
Apr 16, 2013·Analytical Biochemistry·Vesna B JovanovićLjuba M Mandić
Jul 3, 2013·Biochimica Et Biophysica Acta·Wojciech BalPeter Faller
Jul 31, 2013·Clinica Chimica Acta; International Journal of Clinical Chemistry·Jeanethe AnguizolaDavid S Hage

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Citations

Dec 22, 2016·Metallomics : Integrated Biometal Science·Alejandra Sarahi Ramirez SegoviaKatarzyna Wrobel
Nov 21, 2018·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Ana Z PenezićLjuba M Mandić

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