Hsa-microRNA-181a is a regulator of a number of cancer genes and a biomarker for endometrial carcinoma in patients: a bioinformatic and clinical study and the therapeutic implication

Drug Design, Development and Therapy
Shuming HeShu-Feng Zhou

Abstract

The aberrant expression of human microRNA-181a-1 (hsa-miR-181a) has been implicated in the pathogenesis of various cancers, serving as an oncogene or a tumor suppressor. However, the role of hsa-miR-181a in the pathogenesis of endometrial carcinoma (EC) and its clinical significance are unclear. This study aimed to search for the molecular targets of hsa-miR-181a using bioinformatic tools and then determine the expression levels of hsa-miR-181a in normal, hyperplasia, and EC samples from humans. To predict the targets of hsa-miR-181a, ten different algorithms were used, including miRanda-mirSVR, DIANA microT v5.0, miRDB, RNA22 v2, TargetMiner, TargetScan 6.2, PicTar, MicroCosm Targets v5, and miRWALK. Two algorithms, TarBase 6.0 and miRTarBase, were used to identify the validated targets of hsa-miR-181a-5p (a mature product of hsa-miR-181a), and the web-based Database for Annotation, Visualization and Integrated Discovery (DAVID) 6.7 was used to provide biological functional interpretation of the validated targets of hsa-miR-181a-5p. A total of 78 formalin-fixed, paraffin-embedded tissue specimens from 65 patients and 13 healthy subjects were collected and examined, including normal endometrium (n=13), endometrial hyperplasia (...Continue Reading

Citations

Nov 22, 2015·Nucleic Acids Research·Chih-Hung ChouHsien-Da Huang
Oct 1, 2016·Applied Biochemistry and Biotechnology·Zeinab AhsaniMahdi Paryan
Dec 15, 2018·Molecular Oncology·Cornelia BraicuIoana Berindan-Neagoe
Aug 5, 2020·Journal of Clinical Laboratory Analysis·Wenjin XuHuifen Liu
Oct 18, 2019·Frontiers in Oncology·Suraksha TamangAklank Jain
Apr 4, 2021·Cancers·Amélia FavierGeoffroy Canlorbe

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Methods Mentioned

BETA
CLIP-Seq
PCR

Software Mentioned

TargetScan
Vienna
microT
miRWALK
RNAhybrid
MicroCosm
miRDB
MicroCosm Targets
MirTarget2
PicTar

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