hsa_circ_0000177-miR-638-FZD7-Wnt Signaling Cascade Contributes to the Malignant Behaviors in Glioma

DNA and Cell Biology
Zhiqiang Chen, Xingbang Duan

Abstract

As a new member of the noncoding RNA family, circular RNAs (circRNAs) have been demonstrated as critical regulators in various physiological and pathological processes, such as tumorigenesis. However, the role of circRNAs has not been well understood until now. In our study, we found that circRNA hsa_circ_0000177 was upregulated in glioma tissues and cell lines. Also, hsa_circ_0000177 overexpression was associated with poor prognosis in glioma patients. Through functional experiments, we found that hsa_circ_0000177 knockdown dramatically inhibited glioma cell proliferation and invasion in vitro. Consistently, hsa_circ_0000177 knockdown significantly repressed glioma growth in vivo. In terms of mechanism, we used bioinformatics analysis and identified hsa_circ_0000177 as a miR-638 sponge. We showed that miR-638 inhibition could restore the proliferation and invasion of glioma cells transfected with hsa_circ_0000177 small interfering RNA. Furthermore, we demonstrated that frizzled class receptor 7 (FZD7) was targeted by miR-638 and upregulated by hsa_circ_0000177. Through upregulating FZD7 expression, hsa_circ_0000177 activated Wnt signaling and facilitated glioma growth. Taken together, our study revealed a novel signaling pathw...Continue Reading

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Citations

May 23, 2019·Journal of Molecular Neuroscience : MN·Feng ZhangLiang Zhang
Aug 20, 2019·Journal of Experimental & Clinical Cancer Research : CR·Qiang LiuYunjie Wang
Sep 19, 2019·Molecular Cancer·Bing-Qing ShangJin Bai
Nov 22, 2018·Journal of Molecular Neuroscience : MN·Zheng HaoMeihua Li
Feb 7, 2019·Cancer Gene Therapy·Xiaotong ZhangChuize Kong
Apr 23, 2020·International Journal of Oncology·Yuhao ZhangHong Wang
Jun 21, 2020·Signal Transduction and Targeted Therapy·Lei LiuShouping Xu
Feb 18, 2020·Molecular Cancer·Jikui SunJinhuan Wang
Feb 10, 2019·Journal of Experimental & Clinical Cancer Research : CR·Zhenwei HeYixue Xue
Mar 2, 2021·Pathology, Research and Practice·Zhi Xiong ChongWan Yong Ho
Feb 19, 2021·Computational and Structural Biotechnology Journal·Xiaozhu TangChunyan Gu
May 18, 2021·Frontiers in Genetics·Liang CenHongjuan Cui
Jan 3, 2020·Molecular Therapy. Nucleic Acids·Fei JiYan Ding

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Methods Mentioned

BETA
xenografts
transfection
xenograft

Software Mentioned

SPSS
CircInteractome
TargetScan

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