Hsa_circ_0128846 promotes tumorigenesis of colorectal cancer by sponging hsa-miR-1184 and releasing AJUBA and inactivating Hippo/YAP signalling.

Journal of Cellular and Molecular Medicine
Xu WangDi Sun

Abstract

Hsa_circ_0128846 was found to be the most significantly up-regulated circRNA in our bioinformatics analysis. However, the role of hsa_circ_0128846 in colorectal cancer has not been explored. We thus aim to explore the influence and mechanism of hsa_circ_0128846 in colorectal cancer by sponging its downstream miRNA target miR-1184. We collected 40 colorectal cancer patients' tumour tissues to analyse the expression of hsa_circ_0128846, miR-1184 and AJUBA using qRT-PCR and Western blot where needed. Then, we constructed stably transfected SW480 and HCT116 cells to study the influence of hsa_circ_0128846, miR-1184 and AJUBA on colorectal cancer cell phenotypes. To obtain reliable results, a plethora of experiments including RNA immunoprecipitation assay, flow cytometry, EdU incorporation assay, wound healing migration assay, transwell invasion assay and live imaging of nude mice xenograft assay were performed. The binding relationship between hsa_circ_0128846, miR-1184 and AJUBA mRNA in colorectal cancer was validated by reported gene assay. In colorectal cancer tissues, circ_0128846 and AJUBA were both significantly up-regulated, while miR-1184 was significantly down-regulated compared with healthy tissues. Meanwhile, hsa_circ_01...Continue Reading

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Citations

Dec 30, 2020·Experimental Dermatology·Krista Schleicher, Daniel Schramek
Feb 12, 2021·Cancer Management and Research·Jiali WangMeng Ye
May 21, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Soudeh Ghafouri-FardReza Vafaee
Jun 7, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Soudeh Ghafouri-FardMohammad Taheri
Jul 11, 2021·The Kaohsiung Journal of Medical Sciences·Ayiguli HareHai-Feng Wang
Sep 19, 2021·Cancer Cell International·Mandana Ameli-MojaradMaziar Ashrafian Bonab

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Datasets Mentioned

BETA
GSE126095
GSE37178

Methods Mentioned

BETA
electrophoresis
PCR
transfection
Flow cytometry
FACS
immunoprecipitation
RIP
flow
transfections

Software Mentioned

FluorChem FC2
Living Image
TargetScan Human

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