Hsa_circ_0137008 suppresses the malignant phenotype in colorectal cancer by acting as a microRNA-338-5p sponge

Cancer Cell International
Zhanfeng YangZhi Jiang

Abstract

Circular RNAs (circRNAs) have been shown to play a crucial role in tumorigenesis. In this study, we investigated the function of hsa_circ_0137008 and its underlying molecular mechanism in colorectal cancer (CRC). Gene expression was conducted by quantitative real-time PCR or western blot. Functional experiments were performed by cell count kit-8, colony formation assay, wound healing, and transwell assays. Luciferase reporter assay and RNA pull-down assay were performed to investigate the molecular mechanism of hsa_circ_0137008 in CRC. In addition, the xenograft tumor model was applied to determine the role of hsa_circ_0137008 in vivo. Downregulation of hsa_circ_0137008 was observed in CRC tissues and cell lines. Functionally, overexpression of hsa_circ_0137008 inhibited the proliferation of CRC cells, as indicated by the inhibition of proliferative protein expression (Ki67 and PCNA), reduced cell viability and colony formation ability. Upregulation of hsa_circ_0137008 suppressed the migration, invasion, and epithelial to mesenchymal transition (EMT) of CRC cells. Mechanically, hsa_circ_0137008 negatively regulated the expression of microRNA-338-5p (miR-338-5p). Furthermore, hsa_circ_0137008 abated the miR-338-5p mediated promo...Continue Reading

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Citations

Feb 8, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Jasni Viralippurath AshrafEyad Elkord
Apr 9, 2021·Cellular & Molecular Biology Letters·Meysam Moghbeli
May 21, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Soudeh Ghafouri-FardReza Vafaee
Jul 11, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Qian YiWeichao Sun
Jul 1, 2021·Oncology Letters·Mingying Zhang, Shubin Wang

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Methods Mentioned

BETA
transfection
PCR
pull-down
xenograft
pull down

Software Mentioned

Image J
CircInteractome
Statistical Program for Social Sciences ( SPSS )

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