Hsa_circ_RNA_0011780 Represses the Proliferation and Metastasis of Non-Small Cell Lung Cancer by Decreasing FBXW7 via Targeting miR-544a

OncoTargets and Therapy
Yong LiuHanping Shi

Abstract

Circular RNA (circRNA) is involved in the development of various cancers. However, whether circRNA can inhibit the tumorigenesis of non-small cell lung cancer (NSCLC) is still unclear. We aimed to explore the epigenetic function of tumor-suppressive circRNA (hsa_circ_RNA_0011780) and its downstream regulatory factors in NSCLC. Quantitative polymerase chain reaction (qPCR) was used to evaluate hsa_circ_11780 expression in NSCLC tissues and cell lines. The impact of high hsa_circ_11780 expression on overall survival in patients with NSCLC was tested using the Log rank test. The association between decreased hsa_circ_11780 expression and clinicopathological features in patients with NSCLC was analyzed using the Chi-squared test. In vitro cell proliferation and apoptosis were assayed using the cell counting kit-8 (CCK-8) and flow cytometry, respectively. Mice xenograft models were used to determine the tumor promoting effects of hsa_circ_11780 on NSCLC in vivo. The underlying regulatory mechanism was predicted by bioinformatics and verified by a dual-luciferase reporter assay, RNA transfection, qPCR, and Western blotting. The correlation between miR-544a and hsa_circ_11780 expression was verified using Spearman correlation coeffici...Continue Reading

Citations

Jun 11, 2020·Postgraduate Medical Journal·Qian YangYuanshuai Huang
Jan 22, 2021·Environmental Toxicology·Qun LiuGang Li
Sep 2, 2021·Cancer Management and Research·Huanhuan DongHui Xu

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Methods Mentioned

BETA
biopsy
PCR
Assay
Flow Cytometry
RIP
Protein Assay
electrophoresis
transfection
xenograft
immunoprecipitation

Software Mentioned

Image J
TargetScan
GraphPad Prism

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