Abstract
We previously blocked the heat shock transcription factor 1 function with a dominant-negative mutant (mHSF1) in breast cancer cell line Bcap37, and found that mHSF1 sensitizes Bcap37 cells to hyperthermia by promoting the apoptotic process. Here we studied the mechanism of this abolishing process and how thermotolerance develops in Bcap37 cells. The results indicated that mHSF1 abolished acquired or intrinsic thermotolerance in Bcap37 cells by enhancing JNK and caspase-3 pathways, two stress-induced apoptotic pathways, after hyperthermia, and interference with either one of them attenuated hyperthermia-induced apoptosis. Furthermore, epistasis assay of these two pathways suggested that JNK was upstream of the caspase-3 pathway. Conversely, other hyperthermia-induced kinases implicated in cell survival and death, Akt, ERK or p38, did not influence the effect of mHSF1, indicating that these kinases were not implicated in this abolishing process. In addition, we found that the development of acquired thermotolerance of Bcap37 cells was associated with the suppression of JNK activation after mild preheat treatment and was not reduced by Akt, ERK or p38 inhibition. In contrast, the intrinsic thermotolerance of Bcap37 cells was due t...Continue Reading
References
Nov 24, 1995·Science·Z XiaM E Greenberg
Aug 1, 1995·Molecular and Cellular Biology·J ZuoR Voellmy
Apr 1, 1995·The Journal of Clinical Investigation·J C PlumierG N Pagoulatos
Mar 4, 1995·Lancet·J OvergaardS M Bentzen
Sep 22, 1994·Nature·Y A LazebnikW C Earnshaw
Jan 1, 1993·Annual Review of Genetics·D A Parsell, S Lindquist
Mar 7, 1996·Nature·M VerheijR N Kolesnick
Oct 4, 1996·The Journal of Biological Chemistry·J M Kyriakis, J Avruch
May 1, 1996·Current Biology : CB·B W ZankeJ R Woodgett
Mar 1, 1996·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·F F LiuR P Hill
Sep 1, 1997·Molecular and Cellular Biology·D D MosserB Massie
Jun 27, 1998·The Journal of Biological Chemistry·K A BuzzardR L Anderson
Oct 14, 1998·The Journal of Clinical Investigation·G ChoukrounT Force
Feb 6, 1999·International Journal of Cancer. Journal International Du Cancer·I HerrK M Debatin
Mar 30, 1999·Experimental Cell Research·M Jäättelä
Aug 10, 1999·Journal of Cellular Biochemistry·W WoessmannN F Mivechi
Dec 2, 1999·Experimental Cell Research·E S Kandel, N Hay
May 8, 2000·Science·C TournierR J Davis
May 12, 2000·The Journal of Biological Chemistry·C Y LiJ S Seo
Aug 25, 2000·Molecular and Cellular Biology·V L GabaiM Y Sherman
Dec 19, 2000·Human Gene Therapy·V BraidenS Yamashita
Jan 9, 2001·Cell Stress & Chaperones·V VollochM Y Sherman
Feb 24, 2001·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·M H Falk, R D Issels
Feb 1, 2002·Biochemical and Biophysical Research Communications·Jin-Hui WangXin-Yuan Liu
Jan 23, 2003·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·S K Calderwood, A Asea
Citations
Mar 11, 2006·Apoptosis : an International Journal on Programmed Cell Death·M LauricellaG Tesoriere
May 13, 2010·Journal of Translational Medicine·Cheng WangGuangxiu Lu
Feb 3, 2009·Journal of Hematology & Oncology·Mark G Goldstein, Zihai Li
Jan 18, 2013·World Journal of Gastroenterology : WJG·Feng XiaoQing-Xian Zhu
Oct 9, 2012·Pharmacology & Therapeutics·Jong Hun LeeAh-Ng Tony Kong
Feb 22, 2005·Biochemical and Biophysical Research Communications·Shigenori HoshinoIchiro Kawase
May 10, 2005·Journal of Pharmacological Sciences·Takayuki SakaiMitsuru Kawaguchi
Aug 17, 2012·Oncology Reports·Lin WangYiming Wu