HSP22, a new member of the small heat shock protein superfamily, interacts with mimic of phosphorylated HSP27 ((3D)HSP27)

The Journal of Biological Chemistry
R BenndorfMichael J Welsh

Abstract

Most of the members of the superfamily of mammalian small heat shock or stress proteins are abundant in muscles where they play a role in muscle function and maintenance of muscle integrity. One member of this protein superfamily, human HSP27, is rapidly phosphorylated on three serine residues (Ser(15), Ser(78), and Ser(82)) during cellular response to a number of extracellular factors. To understand better the role of HSP27, we performed a yeast two-hybrid screen of a human heart cDNA library for HSP27-interacting proteins. By using the triple aspartate mutant, a mimic of phosphorylated HSP27, as "bait" construct, a protein with a molecular mass of 21.6 kDa was identified as an HSP27-binding protein. Sequence analysis revealed that this new protein shares an overall sequence identity of 33% with human HSP27. This protein also contains the alpha-crystallin domain in its C-terminal half, a hallmark of the superfamily of small stress proteins. Thus, the new protein itself is a member of this protein superfamily, and consequently we designated it HSP22. According to the two-hybrid data, HSP22 interacts preferentially with the triple aspartate form of HSP27 as compared with wild-type HSP27. HSP22 is expressed predominantly in muscl...Continue Reading

References

Dec 31, 1991·Biochemical and Biophysical Research Communications·K N BitarJ R Strahler
Jul 1, 1991·The Journal of Cell Biology·T MironB Geiger
May 10, 1990·Molecular and Cellular Biochemistry·Y L Siow, K Dakshinamurti
Sep 21, 1990·Molecular and Cellular Biochemistry·S LongoniM Chiesi
Dec 18, 1989·FEBS Letters·C E VoorterW W de Jong
Apr 12, 1994·Proceedings of the National Academy of Sciences of the United States of America·M Kantorow, J Piatigorsky
Jan 1, 1995·Cold Spring Harbor Symposia on Quantitative Biology·M J GethingJ Sambrook
Nov 5, 1997·The Journal of Biological Chemistry·R S Piotrowicz, E G Levin
Jul 3, 1998·International Journal of Biological Macromolecules·W W de JongJ A Leunissen
Dec 22, 1998·Biochimica Et Biophysica Acta·W C BoelensW W De Jong
Mar 2, 1999·Biochemical and Biophysical Research Communications·C Liu, M J Welsh
Oct 26, 1999·Cell Structure and Function·K YoshidaS Ohno
Dec 22, 1999·The American Journal of Physiology·C SchäferJ A Williams
Feb 5, 2000·Developmental Biology·S M Davidson, M Morange
Mar 1, 2000·Biochemical and Biophysical Research Communications·K SakamotoT Nagao
Nov 9, 2000·Archives of Biochemistry and Biophysics·J HawkinsP LoGrasso

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Citations

Jan 28, 2005·Cellular and Molecular Neurobiology·Jun-ichi Satoh, Takashi Yamamura
Mar 1, 2005·Journal of Molecular and Cellular Cardiology·Ryan P Taylor, Ivor J Benjamin
Nov 14, 2002·Biochimica Et Biophysica Acta·Olesya O PanasenkoNikolai B Gusev
May 4, 2004·Nature Genetics·Joy IrobiVincent Timmerman
Jan 14, 2004·European Journal of Biochemistry·Olesya V BukachNikolai B Gusev
Dec 2, 2009·The Journal of Biological Chemistry·Renu WadhwaRobert M Tanguay
Jun 12, 2010·Human Molecular Genetics·Joy IrobiVincent Timmerman
Oct 3, 2002·Current Opinion in Critical Care·Giovanna DeloguGiuseppe Famularo
Nov 6, 2002·Journal of Cellular and Molecular Medicine·Dana Perkins
Dec 8, 2010·Molecular and Cellular Biology·Yongsheng ShiJames L Manley
Oct 17, 2002·Cell Stress & Chaperones·Nicole Mounier, André-Patrick Arrigo
Jun 25, 2003·Cell Stress & Chaperones·Guido KappéWilfried W de Jong
Jun 25, 2003·Cell Stress & Chaperones·Jean-Marc FontaineRainer Benndorf
Apr 24, 2012·The International Journal of Biochemistry & Cell Biology·Julie AcunzoPalma Rocchi
Apr 17, 2007·The Journal of Nutritional Biochemistry·Arthur GriderAngus G Scrimgeour
Apr 21, 2007·Cardiovascular Drug Reviews·Ilan J DananChristophe Depre
Aug 2, 2006·Journal of Neurochemistry·Jean-Claude DavidJean-François Grongnet
Oct 18, 2005·Biochemical and Biophysical Research Communications·Jean-Marc FontaineMichael J Welsh
Feb 27, 2004·Biochemical and Biophysical Research Communications·Maria V KimNikolai B Gusev
Nov 16, 2004·Biochemical and Biophysical Research Communications·Maria V KimNikolai B Gusev
Nov 5, 2003·The Journal of Biological Chemistry·Xiankui SunRainer Benndorf
Jan 30, 2004·American Journal of Physiology. Cell Physiology·Sita Somara, Khalil N Bitar
Mar 16, 2018·Journal of Applied Physiology·Jared M DickinsonChad C Carroll
Dec 28, 2007·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Martha MarvinLara D Hutson
Jun 1, 2006·Journal of Clinical Neurology·Jung-Hwa Lee, Byung-Ok Choi
Oct 6, 2005·Journal of Muscle Research and Cell Motility·Olesya V BukachNikolai B Gusev

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