HSP60 plays a regulatory role in IL-1β-induced microglial inflammation via TLR4-p38 MAPK axis

Journal of Neuroinflammation
Shalini SwaroopAnirban Basu

Abstract

IL-1β, also known as "the master regulator of inflammation", is a potent pro-inflammatory cytokine secreted by activated microglia in response to pathogenic invasions or neurodegeneration. It initiates a vicious cycle of inflammation and orchestrates various molecular mechanisms involved in neuroinflammation. The role of IL-1β has been extensively studied in neurodegenerative disorders; however, molecular mechanisms underlying inflammation induced by IL-1β are still poorly understood. The objective of our study is the comprehensive identification of molecular circuitry involved in IL-1β-induced inflammation in microglia through protein profiling. To achieve our aim, we performed the proteomic analysis of N9 microglial cells with and without IL-1β treatment at different time points. Expression of HSP60 in response to IL-1β administration was checked by quantitative real-time PCR, immunoblotting, and immunofluorescence. Interaction of HSP60 with TLR4 was determined by co-immunoprecipitation. Inhibition of TLR4 was done using TLR4 inhibitor to reveal its effect on IL-1β-induced inflammation. Further, effect of HSP60 knockdown and overexpression were assessed on the inflammation in microglia. Specific MAPK inhibitors were used to r...Continue Reading

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Citations

Aug 2, 2016·Journal of Diabetes Research·Joshua Juwono, Ryan D Martinus
Sep 7, 2016·Behavioural Brain Research·Nan-Qu HuangFeng Jin
Mar 21, 2017·Journal of Pharmacological Sciences·Yukimichi NakanoHideaki Hara
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Apr 16, 2021·Nanomedicine : Nanotechnology, Biology, and Medicine·Eric DyneMin-Ho Kim

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Methods Mentioned

BETA
mechanical dissociation
transfection
electrophoresis
PCR
co-immunoprecipitation

Software Mentioned

GeneTools
Search Tool for the Retrieval of
BD CBA
GraphPad Prism
PD Quest
ProteinPilot
Mascot
GeneSnap
Syngene
GeneCodis

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