PMID: 8601336Mar 1, 1996Paper

HSP70-2 is part of the synaptonemal complex in mouse and hamster spermatocytes

Chromosoma
J W AllenE M Eddy

Abstract

Mouse spermatogenic cells are known to express HSP70-2, a member of the HSP70 family of heat-shock proteins. The purpose of the present study was to characterize further the expression and localization of HSP70-2 in meiotic cells of mice and hamsters. After separating mouse spermatogenic cells into cytoplasmic and nuclear fractions, proteins were separated by two-dimensional gel electrophoresis and detected with HSP-specific antibodies. Of several HSP70 proteins identified in the cytoplasm, only HSC70 and HSP70-2 were also detected in the nucleus. Immunocytological analyses of spermatocyte prophase cells revealed that HSP70-2 was associated with the synaptonemal complex. Surface-spread synaptonemal complexes at pachytene and diplotene stages labeled distinctly with the antiserum to HSP70-2. Synaptonemal complexes from fetal mouse oocytes failed to show any evidence of HSP70-2. Reverse-transcriptase-polymerase chain reaction (RT-PCR) analyses of gene expression confirmed this sex specificity; Hsp70-2 mRNA was detected in mouse testes, but not ovaries. These findings are suggestive of a previously unsuspected sexual dimorphism in structure and/or function of the synaptonemal complex.

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Citations

Mar 26, 1999·Molecular Reproduction and Development·N TsunekawaH Fujimoto
May 23, 2009·Histochemistry and Cell Biology·Natalia VydraWieslawa Widlak
Mar 5, 2011·Histochemistry and Cell Biology·Dorota ScieglinskaZdzisław Krawczyk
May 4, 2011·Cell and Tissue Research·Yingjie WuYinghe Qin
May 17, 2008·Journal of Assisted Reproduction and Genetics·M H Nasr-EsfahaniM Tavalaee
Oct 13, 2006·The Journal of Biological Chemistry·Jérôme GovinSaadi Khochbin
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Dec 3, 2005·Reproduction : the Official Journal of the Society for the Study of Fertility·Meisha A Morelli, Paula E Cohen
Feb 5, 2004·The Journal of the Acoustical Society of America·Jean C Krause, Louis D Braida
Aug 6, 2008·The Journal of Cell Biology·Toru SasakiHitoshi Okada
Jun 25, 2008·Parasitology International·Emilio Del CachoCaridad Sánchez-Acedo
Sep 1, 2007·Environmental Toxicology and Pharmacology·Guillaume PelletierIh Chu
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Mar 13, 2020·Genome Biology and Evolution·Amarinder Singh ThindFrancesco Catania

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