hsp70 genes in the human genome: Conservation and differentiation patterns predict a wide array of overlapping and specialized functions.

BMC Evolutionary Biology
Luciano BrocchieriAlberto J L Macario

Abstract

Hsp70 chaperones are required for key cellular processes and response to environmental changes and survival but they have not been fully characterized yet. The human hsp70-gene family has an unknown number of members (eleven counted over ten years ago); some have been described but the information is incomplete and inconsistent. A coherent body of knowledge encompassing all family components that would facilitate their study individually and as a group is lacking. Nowadays, the study of chaperone genes benefits from the availability of genome sequences and a new protocol, chaperonomics, which we applied to elucidate the human hsp70 family. We identified 47 hsp70 sequences, 17 genes and 30 pseudogenes. The genes distributed into seven evolutionarily distinct groups with distinguishable subgroups according to phylogenetic and other data, such as exon-intron and protein features. The N-terminal ATP-binding domain (ABD) was conserved at least partially in the majority of the proteins but the C-terminal substrate-binding domain (SBD) was not. Nine proteins were typical Hsp70s (65-80 kDa) with ABD and SBD, two were lighter lacking partly or totally the SBD, and six were heavier (>80 kDa) with divergent C-terminal domains. We also ana...Continue Reading

References

Nov 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·S Henikoff, J G Henikoff
Jan 1, 1990·Immunogenetics·C M Milner, R D Campbell
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Jan 1, 1994·Journal of Molecular Evolution·W R BoorsteinE A Craig
Apr 1, 1996·Cell Stress & Chaperones·M TavariaR L Anderson
Mar 26, 1998·Journal of Molecular Biology·L Brocchieri, S Karlin
Mar 5, 1999·Science·M W GrayB F Lang
Nov 10, 2000·Biochimica Et Biophysica Acta·A J ParsianC R Hunt
Jun 23, 2001·Molecular Biology and Evolution·B R Bettencourt, M E Feder
Apr 5, 2002·Genome Research·W James Kent
Apr 20, 2002·Journal of Molecular Evolution·Brian R Bettencourt, Martin E Feder
Jun 1, 2002·Ageing Research Reviews·Alberto J L Macario, Everly Conway de Macario
Dec 10, 2002·Experimental Gerontology·Gábor NardaiCsaba Söti
Jan 29, 2003·Proceedings of the National Academy of Sciences of the United States of America·Zhihua HanJan L Breslow
Oct 8, 2003·Systematic Biology·Stéphane Guindon, Olivier Gascuel
Dec 25, 2003·Molecular Biology and Evolution·Nikolas Nikolaidis, Masatoshi Nei
Apr 16, 2004·Molecular Biology and Evolution·Grzegorz KudlaLeszek Lipinski
Dec 21, 2004·Nucleic Acids Research·Pora KimSanghyuk Lee
Apr 5, 2005·Genome Research·Namshin KimSanghyuk Lee
Oct 7, 2005·The New England Journal of Medicine·Alberto J L Macario, Everly Conway de Macario
Jun 10, 2006·Hematology/oncology Clinics of North America·Kelvin P LeeEckhard R Podack
Jul 18, 2006·Current Topics in Medicinal Chemistry·Jeffrey L Brodsky, Gabriela Chiosis
Nov 14, 2006·Nucleic Acids Research·John E KarroMark Gerstein
Nov 25, 2006·Mechanisms of Ageing and Development·Luciano BrocchieriAlberto J L Macario
Nov 28, 2006·Frontiers in Bioscience : a Journal and Virtual Library·Alberto J L Macario, Everly Conway de Macario
May 4, 2007·FEBS Letters·Alberto J L Macario, Everly Conway de Macario
May 8, 2007·Annals of the New York Academy of Sciences·Alberto J L Macario, Everly Conway de Macario
May 9, 2007·Frontiers in Bioscience : a Journal and Virtual Library·Ripudaman SinghSuresh I S Rattan
Dec 6, 2011·Nucleic Acids Research·Scott Federhen

❮ Previous
Next ❯

Citations

Mar 5, 2011·Histochemistry and Cell Biology·Dorota ScieglinskaZdzisław Krawczyk
Apr 8, 2010·Extremophiles : Life Under Extreme Conditions·Shenghao LiuXiaohang Huang
Jul 16, 2008·Marine Biotechnology·Steven RobertsFrederick Goetz
Apr 1, 2010·Molecular Neurobiology·Tom Z LuZhong-Ping Feng
Jul 30, 2008·Cell Stress & Chaperones·Harm H KampingaLawrence E Hightower
Aug 7, 2012·Cell Stress & Chaperones·Ari M ChowIan R Brown
Mar 29, 2013·Animal : an International Journal of Animal Bioscience·E NegratoL Bonfanti
Mar 7, 2013·Nature Reviews. Molecular Cell Biology·Evan T Powers, William E Balch
Nov 28, 2013·Genome Biology and Evolution·Jacek KominekBarry L Williams
Sep 24, 2011·Molecular Biology and Evolution·Lee HenryBernard J Crespi
Jan 20, 2011·Molecular and Cellular Biology·Himjyot JaiswalSabine Rospert
Mar 8, 2012·Molekuliarnaia biologiia·V A ChernikovS E Severin
Sep 26, 2013·The Journal of Clinical Investigation·Rona G GiffardEverly Conway de Macario
Jul 15, 2009·BMC Genomics·David Delima Morais, Paul M Harrison
Sep 17, 2009·BMC Genomics·Amit N Khachane, Paul M Harrison
Jul 31, 2013·PloS One·Chi-Chou ChiuHsou-min Li
Apr 4, 2014·PloS One·Christian AdamRoland Houben
Aug 28, 2012·Current Pharmaceutical Design·Victoria A AssimonJason E Gestwicki
Jul 31, 2013·Cell Stress & Chaperones·Malgorzata KowalczykJan Kowalski
Feb 20, 2014·Life Sciences·Petros BozidisKaterina Antoniou
Jul 18, 2014·Cell Stress & Chaperones·Guillaume MarcionFabrice Neiers
Mar 28, 2013·Critical Reviews in Biochemistry and Molecular Biology·Tslil Ast, Maya Schuldiner
Feb 13, 2016·Cell Stress & Chaperones·Jürgen Radons
Feb 4, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Lucía Fernández del RíoJosé M Villalba
Feb 15, 2015·Transplant Immunology·Sadaf AtarodAnne Mary Dickinson
Apr 2, 2013·Journal of Bioscience and Bioengineering·Vahid JajarmiBahram Kazemi
Feb 22, 2012·Journal of Molecular Biology·Jose F AbisambraChad A Dickey
Apr 19, 2011·Translational Research : the Journal of Laboratory and Clinical Medicine·Giuseppina NovoGiovanni L Volti
Jul 21, 2010·Journal of Molecular and Cellular Cardiology·Michael TranterW Keith Jones
Mar 21, 2015·Scientific Reports·Chelsea McCallisterNikolas Nikolaidis

❮ Previous
Next ❯

Methods Mentioned

BETA
protein folding
NMR

Software Mentioned

Clustal W
PHD
STCH
UCSC
PHYML
AceView
BLAST
ITERALIGN
ECgene
BLAST TBLASTN

Related Concepts

Related Feeds

Amoebiasis

Amoebiasis, infection by the protozoan parasite Entamoeba histolytica, remains a global health problem, despite the availability of effective treatment. Here is the latest research.