HSP70 is selectively over-expressed in the blast cells of the germinal centres and paracortex in reactive lymph nodes

Histopathology
O LeopardiB Frigo

Abstract

We evaluated by immunohistochemistry HSP70 expression in reactive lymph nodes since its morphological expression and location have not been previously described and correlated with lymphocyte kinetics. Ninety-six cases of non-specific lymphadenitis were immunostained for HSP70, CD20, CD3, Ki67, Bcl-2, CD21. The type and the location of HSP70-positive cells were determined. Their number out of 2000 cells in each germinal centre and in each paracortical area was counted at 60x magnification with the help of a quantitative grid. Seventeen percent of germinal centre cells and 7.6% of the paracortex cells were positive. This difference was highly significant. The positively reacting cells were B-cells and had a blast (centroblast or immunoblast) morphology, with negative mantle and marginal lymphocytes and T-cells. Lymphoplasmacytoid cells and plasma cells reacted only weakly or were negative. Germinal centre antigen-presenting cells and interdigitating dendritic cells reacted from lightly to moderately. HSP70 was selectively over-expressed by B-blasts mainly located within germinal centres with a lower number in the paracortex. The difference in the mean number between the two sites was statistically highly significant. No correlat...Continue Reading

References

Jan 2, 1992·Nature·M J Gething, J Sambrook
Mar 11, 1991·FEBS Letters·P M Pechan
Apr 1, 1990·Immunology Today·S H Kaufmann
Jan 1, 1987·Advances in Experimental Medicine and Biology·W J Welch
Nov 1, 1987·The American Journal of Surgical Pathology·P van der Valk, C J Meijer
Jul 1, 1995·Diseases of the Colon and Rectum·A C LazarisB C Golematis
Jan 1, 1995·International Journal of Clinical & Laboratory Research·A J Macario
Jan 1, 1995·Virchows Archiv : an International Journal of Pathology·L C TetlowD E Woolley
Apr 1, 1994·Seminars in Cell Biology·W B Pratt, M J Welsh
Jun 25, 1993·Science·D A Agard
Jan 1, 1993·Annual Review of Cell Biology·C Georgopoulos, W J Welch
Oct 6, 1993·Journal of the National Cancer Institute·D R CioccaS A Fuqua
Apr 7, 1993·Journal of the National Cancer Institute·D R CioccaW L McGuire
Dec 1, 1995·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·J P Hendrick, F U Hartl
Oct 10, 1997·American Journal of Obstetrics and Gynecology·A OtaK Toshimori
Feb 18, 1998·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·T ItoM Kanisawa
Feb 26, 1998·Cell·B Bukau, A L Horwich
Apr 29, 1998·Clinical Immunology and Immunopathology·J GoralJ Clancy
May 20, 1998·Otolaryngology--head and Neck Surgery : Official Journal of American Academy of Otolaryngology-Head and Neck Surgery·R Gandour-EdwardsP J Donald
Aug 26, 1998·International Journal of Cancer. Journal International Du Cancer·C BotzlerG Multhoff
Oct 15, 1998·The Journal of Pathology·K Hollowood, J R Goodlad

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Citations

Oct 29, 2005·BMC Cancer·Francesco CappelloGiovanni Zummo
Oct 4, 2016·Journal of Molecular Histology·Pumza MaganganeRichard Naidoo

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